I-MOPS CAS:1132-61-2 Ixabiso lomenzi

Isiphumo se-MOPS (3-(N-morpholino)propanesulfonic acid) sinxulumene kakhulu namandla ayo okugcina i-pH izinzile. I-MOPS yi-zwitterionic compound, oko kuthetha ukuba iqulethe i-charging kunye ne-negative charge, evumela ukuba isebenze njenge-buffer esebenzayo kwiinkqubo zebhayoloji.

Enye yeendlela eziphambili zokusetyenziswa kwe-MOPS kukukhula kweeseli, apho isetyenziselwa ukugcina i-pH yesixhobo sokukhulisa. Iiseli zifuna i-pH ezinzileyo ukuze zikhule kwaye zisebenze kakuhle, kwaye i-MOPS inceda ekuthinteleni isixhobo sokukhulisa nokuthintela ukuguquguquka kwe-pH okunokuba yingozi kwimpilo yeeseli.

I-MOPS ikwasetyenziswa kakhulu kwiindlela zebhayoloji yemolekyuli ezifana ne-DNA kunye ne-RNA isolation, i-PCR (i-polymerase chain reaction), kunye ne-gel electrophoresis. Kwezi zicelo, i-MOPS inceda ekuzinziseni i-pH yemixube ye-reaction kunye nokusebenzisa ii-buffers, ukuqinisekisa iziphumo ezichanekileyo nezithembekileyo.

Kuhlalutyo lweproteni, i-MOPS ingasetyenziswa njenge-arhente yokuthintela kwiindlela ezinje ngokucocwa kweproteni, ukulinganiswa kweproteni, kunye ne-electrophoresis yeproteni. Inceda ukugcina imeko-bume ye-pH efanelekileyo efunekayo ukuze iproteni izinze kwaye isebenze ngexesha lezi nkqubo.

Ukongeza, i-MOPS ingasetyenziswa kwiimpendulo ze-enzyme kunye nezifundo ze-enzyme kinetics. Amandla ayo okugcina i-buffering avumela ukugcinwa kweemeko ze-pH ezifanelekileyo, nto leyo ebalulekileyo kumsebenzi we-enzyme kunye nokulinganisa ngokuchanekileyo kwe-kinetic.