I-N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine ityuwa yesodium I-CAS:102062-46-4 Umfanekiso obonisiweyo

Ityuwa yesodium ye-N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine CAS:102062-46-4

Ityuwa yesodium ye-N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine yikhemikhali esetyenziswa kakhulu kwizicelo ezahlukeneyo zelebhu. Yinto enyibilikayo emanzini evela kwi-tetramethylbenzidine (TMB) equlathe iqela le-sulfopropyl.

Le khompawundi idla ngokusetyenziswa njengesiseko kwi-colorimetric assays ukuze kufunyanwe ubukho bee-enzymes ezifana nee-peroxidases okanye i-horseradish peroxidase (HRP). Xa ezi enzymes zikhona, zinokuvuselela impendulo nge-N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine sodium salt, nto leyo ekhokelela ekudalweni kwemveliso enemibala. Ubungakanani bombala buhambelana ngqo nomsebenzi we-enzyme, nto leyo evumela ukuba i-enzyme ilinganiswe okanye kufunyanwe ii-substrates ezithile.

Ukunyibilika kwamanzi kwetyuwa yesodium ye-N-(3-Sulfopropyl)-3,3,5,5-tetramethylbenzidine kwenza kube lula ukuyisebenzisa kwizisombululo zamanzi kwaye ihambelana neenkqubo ezahlukeneyo zovavanyo. Iqela layo le-sulfopropyl liphucula ukunyibilika kunye nokuzinza kwekhompawundi, okuvumela ukufunyanwa okusebenzayo nokuthembekileyo kwiimvavanyo ze-enzyme.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

I-Immunohistochemistry (IHC): I-STMB isetyenziswa njenge-chromogenic substrate kwi-IHC ukuze ibone ubukho bee-antigens ezithile kwiisampuli zezicubu. Umbala ophumayo uvumela ukuchongwa kunye nendawo yeeproteni ezijoliswe kuzo.

I-ELISA: I-STMB isetyenziswa kakhulu kwi-ELISA nakwi-enzyme immunoassays (EIA) ukufumanisa nokulinganisa ii-target analytes ezifana neeproteni, iihomoni, kunye nee-antibodies. Impendulo yemibala eququzelelwa yi-STMB inika isignali enokulinganiswa yobukho kunye noxinzelelo lwe-analyte.

I-Western blotting: I-STMB isetyenziswa njengesiseko se-HRP-conjugated secondary antibodies kwi-western blotting ukuze kubonwe ubukho beeproteni ezijoliswe kuzo. Umbala ophumayo uvumela ukufunyanwa kweeproteni ezithile ezinomdla kwi-membrane.

Uvavanyo lomsebenzi we-enzyme: I-STMB ingasetyenziselwa ukulinganisa umsebenzi we-peroxidase wee-enzymes ezahlukeneyo. Ngokujonga ukuveliswa kombala, abaphandi banokumisela umsebenzi we-enzyme baze bavavanye ukusebenza kakuhle kwee-enzyme reactions.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C19H25N2NaO3S
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	102062-46-4
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.