I-N-Ethylmaleimide CAS:128-53-0 Ixabiso lomenzi

I-N-Ethylmaleimide (NEM) yikhompawundi encinci yendalo esetyenziswa kakhulu kuphando lwe-biochemistry kunye ne-molecular biology. Isebenza njengesithinteli esithile samaqela e-protein sulfhydryl (thiol) ngokuguqula nokuthintela ngokungenakuphikiswa umsebenzi wawo. I-NEM isabela kakhulu kumaqela e-sulfhydryl, njengalawo afumaneka kwi-amino acid cysteine, kwaye inokusabela kumaqela e-sulfhydryl akhululekileyo kunye nalawo angaphakathi kwi-protein. Oku kwenza i-NEM ibe sisixhobo esiluncedo sokufunda umsebenzi we-protein, ukusebenzisana kwe-protein-protein, kunye nomsebenzi we-enzyme. Iimpawu zayo zokuthintela zisetyenziswe kwiindlela ezahlukeneyo zokusebenza, kubandakanya i-proteomics, i-enzymology, i-structural biology, kunye nokufunyanwa kwamayeza.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukuguqulwa kweProtheyini: I-NEM iguqula ngokungenakuguquguquka iintsalela ze-cysteine kwiiprotheyini ngokwenza i-covalent adduct. Olu tshintsho lunokuchaphazela umsebenzi weprotheyini, ulwakhiwo, kunye nokusebenzisana. Ukusebenza kwe-NEM namaqela e-sulfhydryl kuyenza ibe sisixhobo esibalulekileyo sokufunda umsebenzi weprotheyini kunye neenkqubo ezixhomekeke kwi-thiol.

Ukuthintelwa kwee-Enzymes: Ii-enzyme ezininzi zixhomekeke kumaqela e-thiol ukuze zenze umsebenzi wazo wokutsala. Ngokusabela kumaqela e-thiol, i-NEM inokuthintela ngokukhethekileyo ii-enzyme ezithile. Oku kuye kwasetyenziswa kakhulu ekufundeni i-enzyme kinetics, ukuqonda iindlela ze-enzymatic, kunye nokuhlola ii-enzyme inhibitors ezinokubakho.

Izifundo zokusebenzisana kweeProteni neeProteni: I-NEM idla ngokusetyenziswa ukuphanda ukusebenzisana kweeproteni neeproteni. Ngokuthintela ngokukhetha iintsalela ze-cysteine ezisebenzayo, i-NEM ithintela ukwakheka kweebhondi ze-disulfide phakathi kweeproteni ezisebenzisanayo, ngaloo ndlela iphazamisa ukusebenzisana kwazo. Oku kuvumela abaphandi ukuba bafunde indima yee-cysteine ezithile kunxibelelwano lweeproteni neeproteni.

Izifundo zoLawulo lweRedox: I-NEM isetyenziswa ekufundeni iindlela zolawulo lwe-redox kwiiproteni. Ngokuguqula ii-cysteines ezisebenzayo, i-NEM inokuzinzisa ukwakheka kweproteni ethile kwaye ithintele i-oxidation engafunekiyo okanye ukunciphisa iintsalela ze-cysteine. Oku kunceda ekuqondeni indima ye-redox signaling kunye neendlela zolawulo ezibuthathaka kwi-redox.

Izifundo zokudibanisa: I-NEM ingasetyenziswa njengearhente yokudibanisa ukuzinzisa ii-protein complexes okanye ukusebenzisana kweeproteni. Ngokuguqula ii-cysteine residues, i-NEM inokudala ii-covalent bonds phakathi kweeproteni ezisebenzisanayo, ivumela abaphandi ukuba baphande ii-protein complexes kunye nezakhiwo zazo. Le ndlela iluncedo ngakumbi kwizifundo zebhayoloji yesakhiwo kunye ne-proteomics.

Ukufunyanwa Kwamayeza: Ngenxa yokukwazi kwayo ukusebenzisana ngokukhethayo neentsalela ze-cysteine, i-NEM isetyenzisiwe kuphando lokufunyanwa kwamayeza. Ingasetyenziswa ukuchonga nokuchonga iithagethi zamayeza ezinokubakho okanye ukufunda ukuchaneka kwamayeza afanelekileyo kwiiproteni ezityebileyo ze-cysteine.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C6H7NO2
Uvavanyo	99%
Inkangeleko	umgubo omhlophe wekristale
Inombolo ye-CAS	128-53-0
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.