I-NBD chloride CAS: 10199-89-0

I-NBD chloride, ekwaziwa ngokuba yi-7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, yidayi ekhanyayo esetyenziswa kakhulu kwiikhemikhali nakwizinto eziphilayo. Ikwiklasi yee-nitrobenzoxadiazole (NBD) derivatives, ezibonisa iimpawu ezinamandla ze-fluorescence.

I-NBD chloride ibonakaliswa kukwazi kwayo ukwenza ii-covalent bonds kunye nee-nucleophiles, ezifana nee-primary amines, ii-thiols, kunye namaqela e-hydroxyl. Le propati ivumela ukuba isetyenziswe njenge-arhente yokubhala iilebula ze-biomolecules, kubandakanya iiproteni, ii-peptides, ii-nucleic acids, kunye nezinye ii-molecules ezincinci.

Xa i-NBD chloride isabela kwi-nucleophile, ifumana i-replacement reaction, nto leyo ebangela ukuba kuvele imveliso ezinzileyo ene-NBD. Le mveliso ine-lebel ibonisa i-fluorescence emission enamandla, nto leyo eyenza ukuba ibe luncedo kwiindlela ezahlukeneyo zokufumanisa kunye nemifanekiso.

Ukukhutshwa kwe-fluorescence kwee-molecules ezineelebheli ze-NBD kunokuvuselelwa kukukhanya kwe-ultraviolet (UV) malunga ne-470 nm, kwaye ukukhanya okukhutshiweyo kudla ngokubonwa malunga ne-530 nm. Olu bude be-emission luwela kummandla oluhlaza we-spectrum ebonakalayo, apho ukuphazamiseka kokufumanisa okuvela kwiisampuli zebhayoloji kuphantsi kakhulu.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukubhala nge-covalent: I-NBD chloride inokwenza ii-covalent bonds kunye namaqela e-nucleophilic kwi-biomolecules, njenge-primary amines, i-thiols, kunye namaqela e-hydroxyl. Oku kuvumela ukubhalwa ngokuthe ngqo kwezi biomolecules ngeenjongo zokufumanisa nokucinga.

Ukuphuculwa kweFluorescence: Xa i-NBD chloride isabela kwi-nucleophiles, yenza imveliso ene-fluorescent NBD-label. Le mveliso ine-lebel ibonisa i-fluorescence emission enamandla, nto leyo eyenza ukuba ibe luncedo ekuboniseni nasekulandeleleni ii-molecule ezine-lebel.

Uvakalelo kunye nokucaciswa: Ukukhutshwa kwe-fluorescence kweemveliso ezineelebheli ze-NBD kubuthathaka kakhulu, okuvumela ukubonwa kwezixa eziphantsi zeemolekyuli ezineelebheli. Ngaphezu koko, ukwakheka kwe-covalent bond phakathi kwe-NBD chloride kunye neenucleophiles kubonelela ngokucaciswa, njengoko kuqinisekisa ukunamathela okuzinzileyo nokucacileyo kwedayi kwi-biomolecule ekujoliswe kuyo.

Ukubhalwa kwe-biomolecule: I-NBD chloride ingasetyenziselwa ukulebhelisha ii-biomolecules ezahlukeneyo, kuquka iiproteni, iipeptides, ii-nucleic acids, kunye nezinye iimolekyuli ezincinci. Oku kwenza abaphandi bakwazi ukuphanda indawo, amandla, kunye nokusebenzisana kwezi biomolecules kwiinkqubo zebhayoloji.

I-Fluorescence microscopy: Ii-biomolecules ezineelebheli ze-NBD zinokubonwa kusetyenziswa i-fluorescence microscopy. Le ndlela ivumela ukujongwa kwee-molecules ezineelebheli kwinqanaba leseli okanye le-subcellular, inika ulwazi oluxabisekileyo malunga nokusasazwa kwazo kunye nokuziphatha kwazo.

I-Flow cytometry: Ii-biomolecules ezineelebheli ze-NBD zinokuhlalutywa kwaye zilinganiswe kusetyenziswa i-flow cytometry. Le ndlela ivumela uhlalutyo oluphezulu lweeseli okanye amasuntswana aneelebheli, inika ulwazi malunga namanqanaba okubonakaliswa okanye iimpawu zomphezulu wee-biomolecules ezineelebheli.

Ukulebhelisha nokulandelela iiproteni: I-NBD chloride ingasetyenziselwa ukulebhelisha ii-amino acid residues ezithile okanye amaqela asebenzayo kwiiproteni. Oku kwenza abaphandi bakwazi ukufunda ulwakhiwo lweproteni, ukugoba, amandla okusebenza, kunye nokusebenzisana ngexesha langempela.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C6H2ClN3O3
Uvavanyo	99%
Inkangeleko	Umgubo otyheli
Inombolo ye-CAS	10199-89-0
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.