I-Nitrotetrazolium Blue chloride CAS:298-83-9

Kuphando lwebhayoloji kunye ne-biochemical, i-Nitrotetrazolium Blue chloride isebenza njengesixhobo esibalulekileyo sokufumanisa nokulinganisa ii-superoxide radicals, ezidlala indima ebalulekileyo kwiinkqubo ezahlukeneyo ze-physiological kunye ne-pathological. Xa isabela nge-superoxide, i-NBT yenza imveliso ye-blue formazan enganyibilikiyo, evumela abaphandi ukuba bahlole ngokubonakalayo ubukho kunye nokusasazwa kwe-superoxide ngaphakathi kweenkcubeko zeeseli, izicubu, okanye izinto eziphilayo ezipheleleyo. Olu buchule lwenza uphando malunga nokubandakanyeka kwe-superoxide radicals kwiziganeko ezahlukeneyo zebhayoloji, kubandakanya ukuhanjiswa kweseli, iindlela zokuzikhusela zomsingathi, kunye nomonakalo we-oxidative. Ukusebenza kweekhemikhali kwe-NBT kunye ne-superoxide radicals kunika ukuqonda ngendima yeentlobo ze-oxygen ezisebenzayo kwimpilo nakwizifo, kubonelela ngeendlela zokuphanda iimeko ezinxulumene noxinzelelo lwe-oxidative ezifana nezifo zentliziyo, iingxaki ze-neurodegenerative, kunye neempendulo zokudumba. Ukongeza, ukusetyenziswa kwe-NBT kufikelela kuvavanyo lokuveliswa kwe-superoxide kwiimodeli zovavanyo lwe-pathologies, okufaka isandla ekuqondeni iindlela zesifo kunye nophuhliso lokungenelela okunokwenzeka okujolise kwiindlela zoxinzelelo lwe-oxidative. Ngaphezu koko, ukusetyenziswa kwe-NBT akupheleli kuphando olusisiseko, njengoko kunempembelelo ekuxilongweni kweklinikhi, ngakumbi kuvavanyo lomsebenzi wenkqubo yomzimba kunye neemeko zokudumba. Ngokubona imveliso ye-superoxide besebenzisa i-NBT, abaphandi kunye neengcali zonyango banokufumana ulwazi olubalulekileyo malunga nemisebenzi yeseli yomzimba kunye neenkqubo ze-oxidative ezingaqhelekanga ezinxulumene nezifo ezahlukeneyo, ngaloo ndlela ziququzelela uvavanyo lokuxilonga kunye nokuxela kwangaphambili. Ukuqukumbela, i-Nitrotetrazolium Blue chloride idlala indima ebalulekileyo kuphando lwebhayoloji kunye ne-biochemical, isebenza njengesixhobo esiguquguqukayo sokufumanisa kunye nokubona ii-radicals ze-superoxide. Amandla ayo okuvelisa i-blue formazan precipitate eyahlukileyo xa isabela nge-superoxide iququzelela uphando malunga noxinzelelo lwe-oxidative, iimpendulo ze-immunological, kunye neemeko zesifo. Ukusetyenziswa kwe-NBT kunceda ekuqondeni ngokunzulu iindima zeentlobo ze-oxygen ezisebenzayo kwimpilo kunye nezifo, kunye nemiphumo enokubakho kuphuhliso lwezixhobo zokuxilonga kunye namaqhinga okunyanga ajolise kwiimeko ezinxulumene noxinzelelo lwe-oxidative. Uphando oluqhubekayo kunye nokusetyenziswa kwe-NBT kuya kunika ulwazi oluthe kratya malunga nokusetyenziswa kwayo kwaye kufake isandla ekuqhubekeni kokuqonda kwethu i-redox biology kunye neenkqubo ezinxulumene ne-pathological.