I-N,N'-Methylenebisacrylamide CAS:110-26-9

I-N,N'-Methylenebisacrylamide (MBAA) yikhemikhali esetyenziswa kakhulu kwi-molecular biology nakwi-biochemistry. Yi-arhente edibanisayo eyongezwa kwiijeli ze-acrylamide ukuze zipolime kwaye zenze i-matrix ezinzileyo ye-electrophoresis.

Ukudibanisa kubhekisa ekwakhiweni kweebhondi zeekhemikhali phakathi kweetyathanga zepolymer, kwaye i-MBAA idlala indima ebalulekileyo kule nkqubo. Xa idityaniswe kwi-acrylamide monomers, i-MBAA izidibanisa ukuze zenze inethiwekhi yeepolymers enobukhulu obuthathu. Olu lwakhiwo oludibeneyo luphucula uzinzo kunye namandla ejeli, okuvumela ukwahlukana kwee-biomolecules ngokusekelwe kubukhulu bazo, itshaja, okanye ezinye iimpawu zomzimba.

I-MBAA isetyenziswa kakhulu kwiindlela ezifana ne-polyacrylamide gel electrophoresis (PAGE) kunye ne-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), esetyenziswa kakhulu kuhlalutyo lweproteni kunye ne-nucleic acid. Inceda ekudaleni i-gel matrix efanayo apho ii-biomolecules zinokuhamba khona ngexesha le-electrophoresis.

Ngaphandle kokusetyenziswa kwayo kwi-gel electrophoresis, i-MBAA ikwafumana ukusetyenziswa kwezinye iindawo ezifana ne-molecular imprinting, i-chromatography, kunye neenkqubo zokuhambisa amayeza. Iyaziwa ngokukwazi kwayo ukudala iinethiwekhi ze-polymer ezizinzileyo neziqinileyo, nto leyo eyenza ukuba ibe sisixhobo esibalulekileyo kuphando nakwiilabhoratri zohlalutyo.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Isiphumo sokudityaniswa kwee-acrylamide: I-MBAA inokudibanisa ii-acrylamide monomers ukuze zenze iinethiwekhi ze-polymer ezidityanisiweyo. Esi siphumo sokudityaniswa kwee-crosslinking siphucula uzinzo kunye namandla ee-gels okanye ii-polymer matrices.

I-Gel Electrophoresis: Enye yezona ndlela zixhaphakileyo zokusetyenziswa kwe-MBAA kukwiindlela ze-gel electrophoresis ezifana ne-polyacrylamide gel electrophoresis (PAGE) kunye ne-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). I-MBAA yongezwa kwi-acrylamide monomers ukuze idibanise kwaye yenze i-gel matrix ezinzileyo, evumela ukwahlukana kwe-biomolecules ngokusekelwe kubukhulu bayo kunye nombane ngexesha le-electrophoresis.

Uhlalutyo lweeProteni: I-MBAA ibalulekile kuphando lweproteni kunye nohlalutyo. Ivumela ukwahlulwa kweeproteni ngobukhulu kwaye ivumela ukufunxwa kwe-Western, ukucocwa kweeproteni, kunye nezinye iindlela zokuchaza iiproteni.

Uhlalutyo lwe-Nucleic Acid: I-MBAA ikwasetyenziswa kwiindlela zohlalutyo lwe-nucleic acid ezifana nokulandelelana kwe-DNA, ukwahlulwahlulwa kwee-DNA fragment, kunye ne-DNA footprinting. Ibonelela nge-gel matrix ezinzileyo eququzelela ukwahlulwa kwee-DNA fragments ngokusekelwe kubukhulu bazo.

Ukuprinta iiMolekyuli: I-MBAA isetyenziswa kwiindlela zokuprinta iiMolekyuli, apho imolekyuli yetemplate ifakwa kwi-polymer matrix. Emva kokususwa kwetemplate, i-polymer matrix igcina inkumbulo yesimo semolekyuli, ivumela ukubopha okukhethiweyo kunye nokuqondwa.

Iinkqubo zokuhambisa amayeza: I-MBAA ingasetyenziselwa ukwenza iinethiwekhi ze-polymer ezidityanisiweyo ezinokukwazi ukukhupha amayeza okanye ezinye iimolekyuli ngendlela elawulwayo. Inezicelo ekudaleni iifomyula ezikhutshwa rhoqo zamayeza, ukuphucula uzinzo lwamayeza, kunye nokuphucula ukusebenza kakuhle kokuhambisa amayeza.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C7H10N2O2
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	110-26-9
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.