I-NSP-SA-NHS CAS: 199293-83-9 Ixabiso lomvelisi

I-NSP-SA-NHS, eyaziwa ngokuba yi-N-succinimidyl S-acetylthioacetate N-hydroxysuccinimide ester, i-compound esetyenziswa ngokuqhelekileyo njenge-thiol-specific crosslinking reagent kwi-bioconjugation reactions.Impembelelo yayo ephambili kukuqulunqwa kweebhondi ze-thioester ezizinzile phakathi kwamaqela e-thiol akhoyo kwii-biomolecules, ezifana neeprotheni okanye iipeptides.

Ukusetyenziswa kwe-NSP-SA-NHS ngokuyinhloko kwintsimi yokuguqulwa kweprotheni kunye nokunyanzeliswa.Ezinye zezicelo zayo eziphambili ziquka:

Iprotheyini ileyibhile: I-NSP-SA-NHS isetyenziselwa ukuncamathisela iilebhile ngokudibeneyo, njengedayi ekhanyayo okanye i-biotin, kwiiproteni okanye iipeptides.Oku kuvumela ukuchongwa, ukuchongwa, kunye nokulandelwa kwee-biomolecules ezinophawu kwiimvavanyo ezahlukeneyo zebhayoloji.

I-protein-protein interactions: I-NSP-SA-NHS ingasetyenziselwa ukuwela iiprotheni ezisebenzisanayo ukuze zifunde ukusebenzisana kweeprotheyini.Oku kudla ngokusetyenziswa kubuchule obufana ne-co-immunoprecipitation okanye ii-assays zokutsalela phantsi ukuchonga amaqabane abophayo okanye ukufunda iiprotein complexes.

I-protein immobilization: I-NSP-SA-NHS ivumela ukuncamathela okuhlangeneyo kweeprotheni okanye iipeptide kwiindawo eziqinileyo, kubandakanywa amaso e-agarose, amaso amagnetic, okanye ii-microplates.Oku kuluncedo kwizicelo ezifana nokucocwa kobudlelwane, ukuhlolwa kweziyobisi, okanye uphuhliso lwe-biosensor.

Ukuguqulwa komphezulu: I-NSP-SA-NHS ingasetyenziselwa ukuguqula umgangatho, njengezilayidi zeglasi okanye i-nanoparticles, kunye neeprotheni okanye i-peptides, ukudala i-biomolecule-functionalized surfaces kwizicelo ezahlukeneyo ezifana nokuxilongwa, iinkqubo zokuhanjiswa kweziyobisi, okanye iiplatifti ze-bio-sensing.