I-4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4

Isiphumo se-4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) kukusebenza njengesiseko se-enzyme beta-glucosidase. Le enzyme iqhekeza i-glucosidic bond ye-MUG, nto leyo ekhokelela ekukhutshweni kwe-4-Methylumbelliferone (4-MU). Ukusetyenziswa kwe-MUG ikakhulu kukwicandelo le-microbiology, ngokukodwa ekufumaneni nasekuchongeni iintsholongwane ezivelisa i-beta-glucosidase. I-MUG isetyenziswa kakhulu ekufumaneni i-Escherichia coli (E. coli) emanzini nakwiisampuli zokutya. I-E. coli ine-enzyme beta-glucosidase, enokuthi i-hydrolyze MUG kwaye ivelise isignali ye-fluorescent xa kukho ukukhanya kwe-ultraviolet (UV). Ipropati ye-fluorescent ye-4-MU ivumela ukuba kube lula ukufumanisa nokulinganisa. Xa i-substrate ye-MUG i-hydrolyzed, i-4-MU eveliswayo ikhupha i-fluorescence eluhlaza okwesibhakabhaka, iququzelela ukuchonga iintsholongwane ezine-beta-glucosidase activity. Le ndlela isetyenziswa kakhulu ekujongeni okusingqongileyo kunye novavanyo lokhuseleko lokutya, njengoko ibonelela ngendlela ekhawulezayo neyobukrelekrele yokufumanisa ungcoliseko lweebhaktheriya. Ukongeza ekusetyenzisweni kwayo kwi-microbiology, i-MUG ingasetyenziselwa ukufunda umsebenzi kunye nokuthintelwa kwe-beta-glucosidase kuphando lwe-biochemistry kunye ne-molecular biology. I-fluorescence yayo ivumela ukulinganiswa kwe-enzyme kinetics kwaye ingasetyenziselwa ukuvavanya abathinteli abanokubakho okanye abaqalisi bomsebenzi we-beta-glucosidase. Ngokubanzi, i-MUG yikhompawundi eguquguqukayo efumana ukusetyenziswa ngokubanzi kwimimandla ye-microbiology, i-biochemistry, kunye ne-molecular biology ekufumaneni umsebenzi we-beta-glucosidase kunye nokuchongwa kweebhaktheriya ezivelisa le enzyme.