I-4-Nitrophenyl Beta-D-glucuronide CAS:10344-94-2

I-4-Nitrophenyl Beta-D-glucuronide yikhemikhali eyenziwe ngokuncamathisela imolekyuli yeglucose kwiqela le-4-nitrophenyl ngokusebenzisa i-glycosidic linkage. Isetyenziswa kakhulu njenge-substrate kwiimvavanyo ze-enzymatic ukufumanisa ubukho kunye nomsebenzi we-β-glucuronidase, i-enzyme ebandakanyeka kwi-metabolism yamayeza ahlukeneyo kunye ne-xenobiotics kwizilwanyana ezincancisayo. Xa i-β-glucuronidase ikhona, iqhekeza i-glycosidic bond phakathi kwe-glucose kunye neqela le-4-nitrophenyl, nto leyo ekhokelela ekukhutshweni kwe-4-nitrophenol, enokufunyanwa nge-spectrophotometric kwi-400-420 nm. Le mpendulo ye-enzymatic inika umlinganiselo wobungakanani bomsebenzi we-β-glucuronidase kwaye idla ngokusetyenziswa njengesixhobo ekufumaneni amayeza, izifundo ze-toxicology, kunye nokuxilongwa kweklinikhi.

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Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukuchongwa komsebenzi we-β-glucuronidase: I-4-NPBG isetyenziswa kakhulu njengesiseko se-chromogenic ukuvavanya ubukho kunye nomsebenzi we-β-glucuronidase kwiisampuli ezahlukeneyo zebhayoloji. Le enzyme inqumla i-glycosidic bond ye-4-NPBG, ivelise i-4-nitrophenol, enokulinganiswa kusetyenziswa i-spectrophotometry.

Izifundo zemetabolism yamayeza: Ekubeni i-β-glucuronidase idlala indima ebalulekileyo kwimetabolism yamayeza kunye ne-xenobiotics, i-4-NPBG ingasetyenziselwa ukuvavanya umsebenzi wale enzyme kwizifundo zemetabolism yamayeza. Inceda ekuqondeni ubungakanani kunye ne-kinetics ye-glucuronidation reactions, ezibalulekileyo ekususweni kwamayeza kunye nokufumaneka kwawo kwi-bioavailability.

Izifundo zeToxicology: Ezinye iikhompawundi zeToxicological zinokuguqulwa zibe yimetabolite kwaye zikhutshwe ngendlela ye-glucuronide conjugates. Ngokusebenzisa i-4-NPBG njenge-substrate, abaphandi banokuhlola umsebenzi we-β-glucuronidase kwiithishu ezahlukeneyo okanye kwiiseli ukuze bavavanye ubuthi obunokubakho okanye iziphumo ezimbi zeekhompawundi.

Ukuxilongwa kwezonyango: Ukulinganiswa komsebenzi we-β-glucuronidase kusetyenziswa i-4-NPBG kungasetyenziswa kwiindawo zonyango ukufumanisa izifo okanye iimeko ezithile. Amanqanaba okanye umsebenzi ongaqhelekanga we-β-glucuronidase unokubonisa iingxaki ezithile zemfuza, ukungasebenzi kakuhle kwesibindi, okanye umhlaza.

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Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C12H13NO9
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	10344-94-2
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.