I-NSP-SA-NHS CAS:199293-83-9 Ixabiso lomenzi

I-NSP-SA-NHS yikhompawundi equlathe i-N-succinimidyl-6-(3′-pyridyldithio)hexanoate (NSP) kunye ne-N-hydroxysuccinimide (NHS). Isetyenziswa kakhulu njenge-arhente yokudibanisa kwiimpendulo ze-bioconjugation, ngokukodwa ekudibaniseni iipeptides, iiproteni, okanye ezinye iimolekyuli eziqulethe i-amine kwiindawo okanye kwezinye iimolekyuli. I-NSP moiety ivumela ukwakheka kwebhondi ye-thioester ezinzileyo kunye nemolekyuli ekujoliswe kuyo, ngelixa i-NHS moiety ivumela ukusabela okulandelayo kunye nee-amine eziphambili. I-NSP-SA-NHS isetyenziswa kakhulu kuphando olwahlukeneyo kunye nezicelo ze-biomedical, kubandakanya ukulungiswa kwe-immunoassays, ukulebhelisha ii-antibody, izifundo zokusebenzisana kweproteni kunye neproteni, kunye nokuthintela ukuhamba kweproteni.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

I-NSP-SA-NHS, ekwaziwa ngokuba yi-N-succinimidyl S-acetylthioacetate I-N-hydroxysuccinimide ester, yikhompawundi esetyenziswa kakhulu njenge-reagent ye-thiol-specific crosslinking kwi-bioconjugation reactions. Isiphumo sayo esiphambili kukwakheka kwezibopho ze-thioester ezizinzileyo phakathi kwamaqela e-thiol akhoyo kwi-biomolecules, njengeeproteni okanye iipeptides.

Ukusetyenziswa kwe-NSP-SA-NHS ikakhulu kukwicandelo lokuguqulwa kweprotheyini kunye nokuthintela ukuhambahamba. Ezinye zezona zicelo zayo ziphambili ziquka:

Ukulebhelisha iiproteni: I-NSP-SA-NHS isetyenziselwa ukuncamathisela iilebheli ngokudibeneyo, ezifana needayi ze-fluorescent okanye i-biotin, kwiiproteni okanye iipeptides. Oku kuvumela ukufunyanwa, ukuchongwa, kunye nokulandelela ii-biomolecules ezilebhelisiweyo kwiimvavanyo ezahlukeneyo zebhayoloji.

Ukusebenzisana kweeproteni neeproteni: I-NSP-SA-NHS ingasetyenziselwa ukudibanisa iiproteni ezisebenzisanayo ukuze kufundwe ukusebenzisana kweeproteni neeproteni. Oku kudla ngokusetyenziswa kwiindlela ezifana ne-co-immunoprecipitation okanye uvavanyo lokutsalwa phantsi ukuze kuchongwe amaqabane abophayo okanye ii-protein complexes zokufunda.

Ukungasebenzi kweeproteni: I-NSP-SA-NHS ivumela ukunamathela kwe-covalent kweeproteni okanye iipeptides kwiindawo eziqinileyo, kubandakanya ii-agarose beads, ii-magnetic beads, okanye ii-microplates. Oku kuluncedo kwizicelo ezinje ngokucocwa kwe-affinity, ukuhlolwa kwamayeza, okanye uphuhliso lwe-biosensor.

Ukuguqulwa komphezulu: I-NSP-SA-NHS ingasetyenziselwa ukuguqula imiphezulu, efana neeslayidi zeglasi okanye ii-nanoparticles, ngeeproteni okanye iipeptides, idala imiphezulu esebenza nge-biomolecule kwizicelo ezahlukeneyo ezifana nokuxilongwa, iinkqubo zokuhambisa amayeza, okanye amaqonga okuva nge-bio.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C32H31N3O10S2
Uvavanyo	99%
Inkangeleko	Umgubo oluhlaza otyheli
Inombolo ye-CAS	199293-83-9
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.