ONPG CAS: 369-07-3 Ixabiso lomvelisi

I-O-nitrophenyl-β-D-galactopyranoside (ONPG) i-substrate yokwenziwa esetyenziswa kwi-biochemical and molecular biology assays ukulinganisa umsebenzi we-enzyme β-galactosidase.Isetyenziswa ngokuqhelekileyo kwiimvavanyo zokubona ukubonakaliswa kofuzo kwiinkqubo zebhaktheriya, ezifana ne-Escherichia coli.ONPG yikhompawundi engenambala ecatshulwe yi-β-galactosidase, ekhokelela ekukhutshweni kwekhompawundi emthubi, i-o-nitrophenol.Umbala otyheli oveliswayo unokulinganiswa nge-spectrophotometrically, ukubonelela ngomlinganiselo ongathanga ngqo womsebenzi we-enzyme.Uvavanyo olusebenzisa i-ONPG luhlala lubizwa ngokuba yi-ONPG assay kwaye yindlela esetyenziswa ngokubanzi kuphando lwebhayoloji ye-molecular ukuvavanya amanqanaba okubonakaliswa kofuzo olulawulwa yi-lac. i-operon kwiiseli zebhaktheriya.

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Impembelelo ye-ONPG njenge-substrate kufuneka icolwe yi-enzyme i-β-galactosidase, ekhokelela ekukhululweni kwemveliso etyheli, i-o-nitrophenol.Olu tshintsho lombala lunokulinganiswa nge-spectrophotometrically, ukuvumela ubungakanani be-β-galactosidase umsebenzi.Ukusetyenziswa kwe-ONPG ngokuyinhloko kuvavanyo lwe-gene expression kwi-molecular biology kunye nophando lwe-microbiology.Ngokuqhelekileyo isetyenziselwa ukulinganisa umsebenzi we-β-galactosidase njengengxelo yezifundo ze-gene expression, ngakumbi kwiibhaktheriya ezifana ne-E. coli.I-gene ye-lacZ, edibanisa i-β-galactosidase, isoloko isetyenziselwa ukuphawula uhlalutyo lwe-gene expression, njengoko ukubonakaliswa kwayo kunokunyanzeliswa ngeemeko ezithile okanye ukulawulwa ngabakhuthazi abathile.Uvavanyo lwe-ONPG lubonelela ngendlela efanelekileyo kunye nethembekileyo yokuvavanya umgangatho ukubonakaliswa kofuzo ngokulinganisa umsebenzi we-β-galactosidase.Olu vavanyo lusetyenziswa ngokubanzi kwizicelo ezahlukeneyo, ezinjengokufunda umsebenzi wokukhuthaza, ukulawulwa kofuzo, kunye nokusebenzisana kweprotein-protein.Ukongezelela, ingasetyenziselwa ukumisela i-enzyme kinetics kunye nokuvavanya imiphumo yokuguquka okanye unyango kumsebenzi we-enzyme.

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Iinkcukacha ezongezelelweyo:

Ukuqamba	C12H15NO8
Isivavanyi	99%
Imbonakalo	Umgubo omhlophe
Inombolo yeCAS.	369-07-3
Ukupakisha	Incinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Ugcino	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	ISO.