Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside CAS:24404-53-3

I-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside isetyenziswa ngokuqhelekileyo njenge-substrate ye-enzyme β-galactosidase.Xa i-β-galactosidase isebenza kuyo, i-compound i-hydrolyzed, ebangela ukuveliswa kwe-p-nitrophenol okanye i-o-nitrophenol, enombala ophuzi onokubonwa lula.Oku kwenza kube luncedo njenge-substrate yovavanyo olulinganisa umsebenzi we-β-galactosidase.

Esinye isicelo esiqhelekileyo sePhenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside sikwi-reporter gene assays.Kolu vavanyo, imfuza yomdla idityaniswa nejeni yentatheli, efana ne-beta-galactosidase gene.Umsebenzi we-reporter gene ke ubekwe iliso ngokulinganisa umsebenzi we-β-galactosidase usebenzisa i-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside njenge-substrate.Oku kuvumela abaphandi ukuba bafunde ukubonakaliswa kofuzo kunye nommiselo.

I-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ingasetyenziselwa ukukhangela i-inhibitors okanye i-activators ye-β-galactosidase.Ngokuvavanya iikhompawundi ezahlukeneyo okanye abaviwa beziyobisi, abaphandi banokuchonga iimolekyuli eziguqula umsebenzi wale enzyme, ezinokuthi zibe nezicelo zonyango kwizifo okanye iimeko ezithile.

Ukongezelela, le ngqungquthela ingasetyenziselwa ukuhlanjululwa kweeprotheni.I-β-galactosidase ihlala idityanisiwe kwiiprotheni ezinomdla ukuze ziququzelele ukubonwa kunye nokucocwa kwazo.I-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ingasetyenziselwa njenge-substrate kwisikrini kwaye ihlambulule ezi proteni ze-fusion ngokufumanisa umsebenzi we-β-galactosidase.