I-Tris(hydroxymethyl)nitromethane CAS:126-11-4

Isiphumo:

Umthamo Wokubhafa: I-Tris isebenza njengearhente esebenzayo yokubhafa ngenxa yokukwazi kwayo ukwamkela okanye ukunikela ngeeproton, igcina uluhlu lwe-pH oluzinzileyo kwizisombululo. Isetyenziswa ngokuqhelekileyo njengesakhi esiphambili kwiinkqubo ze-buffer ukuzinzisa i-pH yeesampuli zebhayoloji kunye neempendulo.

Izicelo:

I-Molecular Biology: I-Tris isetyenziswa kakhulu njenge-arhente yokukhusela kwiindlela ezahlukeneyo ze-molecular biology, kubandakanya i-DNA kunye ne-RNA isolation, i-PCR, i-gel electrophoresis, kunye nokuhlanjululwa kweproteni. Inceda ukugcina imeko-bume ye-pH ezinzileyo, ivumela iimeko ezifanelekileyo zokuphendula kwe-enzyme kunye nokusebenzisana kwe-molecular.

Inkcubeko yeSeli: I-Tris idla ngokusetyenziswa kwimidiya yenkcubeko yeseli ukugcina i-pH eqhubekayo kunye nebhalansi ye-osmotic, ixhasa ukukhula kweeseli ezisempilweni kunye nokuphila kakuhle.

I-Protein Chemistry: I-Tris isetyenziswa kwiimvavanyo ze-protein chemistry, ezifana nokunyibilika kweproteni, uvavanyo lozinzo lweproteni, kunye nezifundo zokubopha iproteni-ligand. Inceda ukugcina uluhlu lwe-pH olufunekayo, ukuqinisekisa ukugoba kunye nokusebenza ngokufanelekileyo kweproteni.

I-Enzymology: I-Tris isetyenziswa kwiimvavanyo ezahlukeneyo ze-enzymatic ukuze kuphuculwe iimeko ze-pH ezifunekayo kumsebenzi we-enzymatic. Iqinisekisa iziphumo ezithembekileyo neziphindaphindwayo, ivumela ukulinganiswa ngokuchanekileyo kwe-enzyme kinetics kunye nezifundo zokuthintela.

Uvavanyo lweBiochemical: I-Tris isetyenziswa njengenxalenye kwiimvavanyo ezininzi ze-biochemical ngenxa yeempawu zayo zokubopha. Igcina i-pH engaguqukiyo ngexesha lovavanyo lwe-colorimetric, spectrophotometric, kunye ne-enzymatic, nto leyo ephucula ukuchaneka nokuthembeka kweziphumo.