Phenylgalactoside CAS: 2818-58-8

Impembelelo kumsebenzi we-enzyme: I-Phenylgalactoside isetyenziswa ngokuqhelekileyo ukulinganisa umsebenzi we-enzyme β-galactosidase.Xa i-phenylgalactoside i-hydrolyzed yi-β-galactosidase, ikhupha i-p-nitrophenol.Ukuqokelela kwe-p-nitrophenol kunokulinganiswa ngokobungakanani, ukubonelela ngengqiqo kumsebenzi we-β-galactosidase.Esi siphumo sisetyenziswe kwizicelo ezifana novavanyo lwe-enzyme kunye neenkqubo zokuhlola.

Uhlalutyo lwe-Gene expression: I-Phenylgalactoside isoloko isetyenziswa njenge-substrate kwiimfuniselo zebhayoloji ye-molekyuli yokufunda ukuchazwa kofuzo.Ijini ye-lacZ, enekhowudi ye-β-galactosidase, idla ngokudityaniswa nolandelelwano lolawulo lwezinye iintlobo zomdla.Ukubonakaliswa kofuzo lwe-lacZ kunye ne-hydrolysis ye-phenylgalactoside nge-β-galactosidase ingabonisa ipateni yokubonakalisa kunye nenqanaba le-gene ekujoliswe kuyo efundwayo.

Iinkqubo zokuhlola: I-Phenylgalactoside yinxalenye ebalulekileyo yeenkqubo zokuhlola ezisebenzisa umsebenzi we-β-galactosidase.Umzekelo omnye owaziwa ngokubanzi yindlela yokuhlola eluhlaza okwesibhakabhaka-mhlophe, esetyenziselwa ukuchonga iiseli eziphinda-phindaneyo okanye eziguquliweyo kumfuniselo webhayoloji yemolekyuli.Iikholoni eziye zathatha ngempumelelo i-DNA edibeneyo okanye i-genetic recombination iya kubonisa i-β-galactosidase, ekhokelela kwi-hydrolysis ye-phenylgalactoside kunye nokwakhiwa kombala ohlaza okwesibhakabhaka.

Ukucocwa kweeprotheyini: Kwezinye iimeko, i-phenylgalactoside ingasetyenziselwa njenge-ligand ye-affinity chromatography ukucoca iiprotheni ezibophelela ngokuthe ngqo okanye zisebenze yi-β-galactosidase.Iprotheyini enomdla inokuba nethegi ye-affinity okanye i-fusion tag ene-β-galactosidase-binding domain.Ngokugqithisa umxube weprotheyini kwikholamu ene-phenylgalactoside engashukumiyo, iprotein efunekayo inokugcinwa ngokukhethiweyo kwaye emva koko ikhutshwe.