I-Phenylgalactoside CAS:2818-58-8

Impembelelo kumsebenzi we-enzyme: I-Phenylgalactoside isetyenziswa kakhulu ukulinganisa umsebenzi we-enzyme i-β-galactosidase. Xa i-phenylgalactoside i-hydrolyzed yi-β-galactosidase, ikhupha i-p-nitrophenol. Ukuqokelelwa kwe-p-nitrophenol kunokulinganiswa ngokobungakanani, okubonelela ngolwazi ngomsebenzi we-β-galactosidase. Esi siphumo sisetyenziswa kwizicelo ezifana novavanyo lwe-enzyme kunye neenkqubo zokuhlola.

Uhlalutyo lokubonakaliswa kwezakhi zofuzo: I-Phenylgalactoside idla ngokusetyenziswa njengesiseko kwizilingo zebhayoloji yemolekyuli ukuze kufundwe ukubonakaliswa kwezakhi zofuzo. I-lacZ gene, efaka i-β-galactosidase, idla ngokudityaniswa nolandelelwano lolawulo lwezinye izakhi zofuzo ezinomdla. Ukubonakaliswa kwezakhi zofuzo ze-lacZ kunye ne-hydrolysis ye-phenylgalactoside yi-β-galactosidase kunokubonisa ipateni yokubonakaliswa kunye nenqanaba lezakhi zofuzo ekujoliswe kuzo ezifundwayo.

Iinkqubo zokuhlola: I-Phenylgalactoside yinxalenye ebalulekileyo yeenkqubo zokuhlola ezisebenzisa umsebenzi we-β-galactosidase. Umzekelo omnye owaziwayo yindlela yokuhlola eluhlaza okwesibhakabhaka, esetyenziselwa ukuchonga iiseli eziphinda-phindayo okanye eziguquliweyo kwiimvavanyo zebhayoloji yemolekyuli. Iikholoni eziye zathatha ngempumelelo i-DNA ephinda-phindayo okanye eziye zafumana i-genetic recombination ziya kuveza i-β-galactosidase, nto leyo ekhokelela kwi-hydrolysis ye-phenylgalactoside kunye nokwenziwa kombala oluhlaza okwesibhakabhaka.

Ukucocwa kweproteni: Kwezinye iimeko, i-phenylgalactoside ingasetyenziswa njenge-ligand ye-affinity chromatography ukucoca iiproteni ezibopha ngokukodwa okanye ezivuselelwa yi-β-galactosidase. Iproteni enomdla inokuba ne-affinity tag okanye i-fusion tag equlethe i-β-galactosidase-binding domain. Ngokudluliswa komxube weproteni kwikholamu ene-phenylgalactoside engashukumiyo, iproteni efunekayo inokugcinwa ngokukhetha kwaye emva koko ikhutshwe.