I-PMSF CAS:329-98-6 Ixabiso lomenzi

Isiphumo:

I-PMSF sisithinteli se-serine protease esibopha ngokungenakuguquguquka kwindawo esebenzayo ye-serine proteases, ngaloo ndlela sithintela umsebenzi wazo.

Itshintsha iintsalela ze-serine ngokusebenzisa ii-covalent bonds, nto leyo ekhokelela ekungasebenzini kwee-enzyme.

I-PMSF inokukhusela iiproteni ekuwohlokeni ngokuthintela umsebenzi weprotease, ngakumbi iiprotease ze-serine.

Izicelo:

Uphando lwe-biochemical: I-PMSF isetyenziswa rhoqo ngexesha lokucocwa kweproteni kunye neenkqubo zokwahlulwa kweproteni ukuthintela ukubola kweproteni. Inceda ukugcina ukuthembeka kunye nozinzo lweproteni ekujoliswe kuyo.

Ukuthintela i-enzyme: I-PMSF sisithako esibalulekileyo sokuthintela i-serine proteases ngexesha lovavanyo lwe-enzyme okanye izifundo ze-biochemical. Ngokuthintela umsebenzi wezi proteases, abaphandi banokuphanda indima ye-serine proteases kwiinkqubo ezahlukeneyo zebhayoloji.

Izifundo zeProteolysis: I-PMSF ingasetyenziselwa ukulawula okanye ukulungisa umsebenzi weprotease kwi-lysates zeseli okanye kwi-extracts. Oku kuluncedo ekufundeni iziganeko zeproteolytic ezibandakanyeka kwiinkqubo zeseli okanye xa i-proteolysis kufuneka ilawulwe kulungiselelo lovavanyo.

Ukuthintela i-protease ngokubanzi: Nangona i-PMSF ijolise ngokukodwa kwi-serine proteases, inokuba nefuthe elithile ekuthinteleni ezinye ii-cysteine ​​proteases. Isetyenziswa njengesithinteli se-protease ngokubanzi xa usebenza ngeesampuli zeproteni ukunciphisa ukubola kwe-proteolytic.