I-Proteinase K ye-albhamu ye-tritirachium CAS:39450-01-6

I-Proteinase K yi-enzyme ekhutshwa kwi-albhamu ye-fungus i-Tritirachium. Yi-serine protease ebanzi, oko kuthetha ukuba inokwahlukanisa iibhondi ze-peptide kwiintlobo ngeentlobo zeeproteni. I-Proteinase K yaziwa ngokukwazi kwayo ukonakalisa iiproteni ngokufanelekileyo, nto leyo eyenza ukuba ibe sisixhobo esibalulekileyo kwiindlela ezahlukeneyo zokusebenza.

Enye yezona ndlela ziphambili zokusetyenziswa kweProteinase K kuphando lwebhayoloji yemolekyuli. Isetyenziswa kakhulu kwiiprotokholi zokukhupha iDNA kunye neRNA ukususa iiproteni ezinokuphazamisa uhlalutyo olusezantsi. IProteinase K inceda ekuguquleni iiseli kwaye yonakalise iiproteni zeseli, nto leyo evumela ukwahlulwa kwee-nucleic acids ezikumgangatho ophezulu.

I-Proteinase K ikwasetyenziswa kakhulu kwizifundo zohlalutyo lweeproteni kunye nokuchonga iimpawu. Ingasetyenziselwa ukugaya iiproteni, ivumela ukuveliswa kweziqwenga zepeptide ezinokuhlalutywa ngeendlela ezifana ne-mass spectrometry. Ukongeza, i-Proteinase K iluncedo ekulandeleni iiproteni kunye nokuchonga indawo yee-amino acids ngaphakathi kwekhonkco le-polypeptide.

Enye indlela ebalulekileyo yokusebenzisa iProteinase K kukucoca umphezulu. Isetyenziswa kakhulu kwiilabhoratri zebhayoloji yemolekyuli ukususa ii-nucleic acids, iiproteni, kunye nezinye izinto ezingcolisayo kwizixhobo zelebhu kunye nomphezulu. IProteinase K izinzile kakhulu kwaye isebenza phantsi kweemeko ezahlukeneyo, kubandakanya amaqondo obushushu aphezulu kunye namanqanaba e-pH ahlukeneyo.