I-Puromycin dihydrochloride CAS: 58-58-2 Ixabiso lomenzi

I-Puromycin dihydrochloride yi-antibiotic enamandla esetyenziswa kakhulu kwi-molecular biology kunye nophando lwe-cell biology. Ithintela ukwenziwa kweproteni ngokubangela ukupheliswa kwangaphambi kwexesha kokwenziwa kweproteni ngexesha lokuguqulwa. I-Puromycin isebenza ngokulinganisa isiphelo se-3' se-aminoacylated tRNA molecule kwaye ifakwe kwikhonkco leproteni elikhulayo. Oku kukhokelela ekunqunyulweni kweproteni kwaye ekugqibeleni kumise inkqubo yokuguqulwa.

Eyona ndlela iphambili yokusebenzisa i-puromycin dihydrochloride kukukhetha nokwahlukanisa iiseli eziguqulwe ngokwezakhi zofuzo okanye ezifakelwe i-puromycin resistance gene. Oku kufezekiswa ngokungenisa i-gene enomdla kunye ne-puromycin resistance gene kwiiseli, uze emva koko uzifake kunyango lwe-puromycin. Ziiseli kuphela eziye zadibanisa ngempumelelo i-puromycin resistance gene kwi-genome yazo eziya kusinda kwaye zande, nto leyo evumela ukukhethwa nokwahlukanisa iiseli ezifunwayo.

I-Puromycin dihydrochloride ikwasetyenziswa kwizifundo ezibandakanya ukubonakaliswa kwezakhi zofuzo kunye nokuveliswa kweproteni. Ngokuthintela ukwenziwa kweproteni, inokunceda abaphandi bafunde indlela esebenza ngayo iproteni, ichonge iiproteni ezithile eziveliswa ngokukhawuleza ukuphendula kwizinto ezithile, kwaye ihlalutye iindlela zokuguqulela.

Ngaphezu koko, i-puromycin dihydrochloride isetyenziselwa ukuvelisa imigca yeseli ezinzileyo enotshintsho oluthile lwemfuza. Ngokubandakanya i-puromycin resistance gene kunye notshintsho lwemfuza olujoliswe kuyo, abaphandi banokuvelisa imigca yeseli eveza ngokuzinzileyo i-gene enomdla kunye nokumelana ne-puromycin. Oku kuvumela izifundo zexesha elide kunye nokuchazwa komsebenzi we-gene.