I-RNase A evela kwi-pancreas yenkomo i-CAS:9001-99-4

I-RNase A, eyaziwa ngokuba yi-ribonuclease A, yi-enzyme ephuma kwi-pancreas yezilwanyana zenkomo (iinkomo). Yiproteni encinci ejolise ngokukodwa kwaye yonakalise iimolekyuli ze-RNA ezikhoyo kwiiseli. I-RNase A isetyenziswa rhoqo kuphando lwebhayoloji yemolekyuli ukususa okanye ukutshabalalisa i-RNA kwiisampuli ze-DNA ukuze kufundwe kwaye kulawulwe i-DNA ngaphandle kokuphazamiseka kwi-RNA. Ikwasetyenziswa kwiinkqubo zokucoca i-RNA, ezinje ngokukhupha i-RNA kunye nokulandelelana kwe-RNA, ukususa ungcoliseko lwe-RNA kunye nokufumana iisampuli ze-RNA ezicocekileyo. I-RNase A inobungcali obuphezulu kwi-RNA ene-strand enye kwaye isebenza kakuhle phantsi kweemeko ze-asidi ezithambileyo. Isetyenziswa kakhulu kwiindlela ezahlukeneyo zelebhu kunye nokusetyenziswa okubandakanya ii-nucleic acids.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukubola kwe-RNA: I-RNase A isetyenziswa rhoqo ukubola okanye ukususa i-RNA kwiisampuli ze-DNA. Le enzyme ijolise ngokukodwa kwi-RNA enemicu enye kwaye iyiqhekeze ibe ziingceba ezincinci. Ingongezwa kwiimpendulo zovavanyo ukuze kususwe iimolekyuli ze-RNA ezingafunekiyo kwaye kugxilwe ekufundweni kwe-DNA.

Ukukhutshelwa kwe-RNA: I-RNase A isetyenziswa kwiiprotokholi zokukhupha i-RNA ukususa ungcoliseko lwe-RNA kwiisampuli ze-DNA okanye zeproteni. Umsebenzi wayo unceda ekufumaneni iisampuli ze-RNA ezicocekileyo zezicelo ezisezantsi ezifana nokubhalwa kwe-reverse, ukulandelelana kwe-RNA, kunye nohlalutyo lokubonakaliswa kwezakhi zofuzo.

Ukucocwa kwe-RNA: Ngexesha lokucocwa kweesampuli ze-RNA, unyango lwe-RNase A ludla ngokwenziwa ukususa intsalela ye-DNA okanye iiproteni ezingcolisayo. Eli nyathelo liqinisekisa ubunyulu kunye nokuthembeka kwe-RNA, nto leyo ebalulekileyo kuhlalutyo oluchanekileyo oluya ezantsi.

Ukuthintelwa kwe-RNA: Nangona i-RNase A ijolise kwaye iyonakalisa i-RNA, ingasetyenziselwa ukuthintela umsebenzi weemolekyuli ezithile ze-RNA ngendlela elawulwayo. Ngokongeza i-RNase A kwiimpendulo okanye iimvavanyo ezithile ezisekelwe kwi-RNA, abaphandi banokuguqula okanye bacinezele umsebenzi we-RNA.

Kwi-Situ Hybridization: RNase A unyango lusetyenziswa kwiindlela ze-in situ hybridization ukususa i-RNA engabotshwanga ngaphambi kokuba ifunyanwe. Oku kuqinisekisa ukuba kuphela iimolekyuli ezithile ze-RNA ezixutywe ngee-probes ezongezelelweyo ezibonwa ngokuchanekileyo.

Ukuzinza kwe-Enzyme: I-RNase A yaziwa ngokuba nozinzo oluqhelekileyo, kwaye umsebenzi wayo ugcinwa phantsi kweemeko ezahlukeneyo zovavanyo. Isetyenziswe njengeprotheyini yomzekelo yokufunda ukugoba kweprotheyini, uzinzo, kunye nomsebenzi we-enzymatic kwizifundo ezahlukeneyo ze-biophysical.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	N / A
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	9001-99-4
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.