I-Streptavidin evela kwi-Streptomyces avidinii CAS:9013-20-1

Ubudlelwane obudibeneyo: I-Streptavidin inobudlelwane obuphezulu kakhulu kwi-biotin, nto leyo eyenza ukuba ibe sisixhobo esihle kakhulu sokubamba nokubona iimolekyuli ze-biotinylated.

Ukucocwa kweeproteni: I-Streptavidin idla ngokusetyenziswa njengesixhobo sokucoca kwi-biotechnology. Iithegi ze-biotinylated okanye iimolekyuli zinokuncamathiselwa kwiibhead ze-streptavidin, okuvumela ukwahlulwa nokucocwa kweeproteni ezithile kwimixube enzima.

Uvavanyo lokufumanisa: I-Streptavidin isetyenziswa kakhulu kwiimvavanyo zokuzikhusela komzimba, ezifana ne-enzyme-linked immunosorbent assays (ELISA), apho inokubopha ii-antibodies okanye ii-antigens ezifunyenwe yi-biotinylated. Oku kuvumela ukufunyanwa okunobunono nokuthe ngqo kweeproteni ezahlukeneyo okanye ii-biomarkers.

Ukuhanjiswa kwamayeza ekujoliswe kuwo: I-Streptavidin ingasetyenziswa kwiinkqubo zokuhanjiswa kwamayeza ngokuyidibanisa neearhente zonyango okanye ii-nanoparticles. Iimolekyuli ekujoliswe kuzo ze-biotinylated zinokudityaniswa ne-streptavidin, nto leyo eququzelela ukuhanjiswa kwamayeza kwiiseli ezithile okanye kwizicubu ezithile.

Iindlela zebhayoloji yeMolekyuli: I-Streptavidin isetyenziswa kwiindlela ezahlukeneyo zebhayoloji yeMolekyuli, ezifana novavanyo lwe-nucleic acid hybridization kunye ne-polymerase chain reaction (PCR). Iiprobes zeBiotinylated okanye ii-primers zingasetyenziselwa ukubona okanye ukukhulisa ii-nucleic acids ezithile, kwaye i-streptavidin emva koko isetyenziselwa ukubona okanye ukubamba iimveliso ze-biotinylated.

Ukufota: I-Streptavidin idla ngokusetyenziswa njengesixhobo kwi-fluorescence microscopy kunye neendlela zokufota. Ngokudibanisa iidayi ze-fluorescent ne-streptavidin, iithagethi ezithile zinokulebhilwa kwaye zibonwe ngobuchule obuphezulu kunye nesisombululo.

Uxilongo: Uvavanyo olusekelwe kwiStreptavidin lusetyenziswa kakhulu ekuxilongweni kwezonyango ukuze kufunyanwe izifo okanye iintsholongwane. Iiprobe zeBiotinylated zinokubona ii-nucleic acids okanye ii-antigens ezithile, nto leyo evumela ukuxilongwa nokubekwa esweni kweemeko ezahlukeneyo.