I-SYBR GREEN I Nucleic Acid Gel Stain CAS:163795-75-3

I-DNA gel electrophoresis: I-SYBR Green I stain yongezwa kwi-DNA gel emva kwe-electrophoresis, kwaye xa ivezwa kukukhanya kwe-UV, inamathela kwiibhendi ze-DNA, ivelise ukukhanya okukhanyayo. Le ndlela yokufaka imibala inceda ekuboniseni nasekuhlalutyeni iziqwenga ze-DNA ezahlulwe ngobukhulu.

I-Quantitative PCR (qPCR): I-SYBR Green I isetyenziswa rhoqo kwi-qPCR ukujonga ukwanda kwe-DNA okanye i-RNA sequences ngexesha langempela. Idayi ibopha kwi-DNA enemicu emibini eveliswa ngexesha lokwandiswa kwe-PCR, kwaye ukwanda kobunzulu be-fluorescence kulinganiswa kumjikelo ngamnye wokwandiswa, okuvumela ukulinganiswa kwethempleyithi ye-nucleic acid yokuqala.

I-RNA gel electrophoresis: I-SYBR Green I ingasetyenziselwa i-RNA gel electrophoresis, okuvumela ukubonakala kunye nohlalutyo lweziqwenga ze-RNA. Iluncedo kuvavanyo lokuthembeka kwe-RNA kunye nokufunda amanqanaba okubonakaliswa kwe-RNA.

Ukuchonga nokuchonga iilebhile ze-nucleic acid: I-SYBR Green I ingasetyenziselwa ukulebhile ii-nucleic acids nge-fluorescence. Oku kunokufezekiswa ngokufaka idayi ngexesha lokwenziwa kwe-nucleic acid okanye ngokusebenzisa iindlela zokubhala iilebhile emva kokubhala. Ii-nucleic acids ezilebhile zinokubonwa kwaye zifunyanwe kusetyenziswa iinkqubo zomfanekiso we-fluorescent.