I-Trichloroaceticacid, ityuwa yesodiyam I-CAS: 650-51-1

I-asidi ye-Trichloroacetic, ityuwa yesodiyam, isebenza indima ebalulekileyo kwiindlela ezahlukeneyo zokusetyenziswa kwicandelo lebhayoloji yemolekyuli, i-biochemistry, kunye ne-protein chemistry. Njenge-reagent yokunciphisa iproteni, isetyenziswa kakhulu kwiindawo zelebhu zokwahlula nokucocwa kweeproteni kunye nee-nucleic acids kwiisampuli zebhayoloji ezintsonkothileyo. Kwi-biology yemolekyuli kunye nophando lwe-biochemistry, ukusetyenziswa kwe-asidi ye-trichloroacetic, ityuwa yesodiyam, kwenza ukuba iiproteni zikwazi ukuhluthwa ngokukhetha kwii-lysates zeseli, ii-supernatants zenkcubeko, kunye nezinye ii-biofluids. Le nkqubo inceda ekususweni kwezinto ezingcolisayo kunye nee-biomolecules ezingafunekiyo, ivumela abaphandi ukuba bafumane iisampuli zeproteni ezicociweyo zohlalutyo olusezantsi olufana ne-electrophoresis, i-Western blotting, i-mass spectrometry, kunye novavanyo lwe-enzyme. Ngaphezu koko, amandla etyuwa okunciphisa ii-nucleic acids ayenza ibe sisixhobo esibalulekileyo sokukhupha nokucocwa kwe-DNA kunye ne-RNA kwizinto zeseli. Ngokwahlula ngokukhethekileyo ii-nucleic acids kwezinye izinto zeseli, i-asidi ye-trichloroacetic, ityuwa yesodiyam, inegalelo ekuhlulweni kwezinto zemfuza ezikumgangatho ophezulu, ezibalulekileyo ekuqokeleleni ii-molecular, ukulandelelana, ukwandiswa kwe-PCR, kunye nezinye iindlela zebhayoloji yemolekyuli. Ukongeza kwindima yayo ekucoceni iiproteni kunye ne-nucleic acid, i-trichloroacetic acid, ityuwa yesodium, isetyenziswa kuphononongo lweendlela zokubonisa iiseli, utshintsho emva kokuguqulwa, kunye nokusebenzisana kweeproteni kunye neeproteni. Ukusetyenziswa kwayo ekulungiseleleni isampuli kunye nokuhlukaniswa kweeproteni kubalulekile ekuhlalutyeni iinkqubo ezintsonkothileyo zebhayoloji kunye nokuqonda imisebenzi yee-biomolecules nganye kwiinkqubo eziphilayo. Ngokubanzi, i-trichloroacetic acid, ityuwa yesodium, idlala indima ebalulekileyo kwimisebenzi yovavanyo lwee-molecular biologists, ii-biochemists, kunye nee-protein chemists, okuvumela ukwahlulwa kunye nokucocwa ngokufanelekileyo kweeproteni kunye nee-nucleic acids kwimithombo eyahlukeneyo yebhayoloji. Iimpawu zayo zokukhetha imvula kunye nokunyibilika kwamanzi kuyenza ibe yinto exabisekileyo yokuphucula uphando lwesayensi, ukufunyanwa kwamayeza, kunye nophuhliso lwezicelo ze-biotechnology ezijolise ekucaciseni ubunzima bomsebenzi weseli kunye nokucutshungulwa kolwazi lwezakhi zofuzo.