I-TRIS-Acetate CAS: 6850-28-8 Ixabiso lomvelisi

I-TRIS-Acetate, sisithintelo esiqhele ukusetyenziswa kwimifuniselo yebhayoloji neyemichiza.Kukudityaniswa kwesiseko se-Tris kunye ne-acetic acid, okubangelwa isisombululo se-pH-stable esetyenziselwa ukulawula nokugcina uluhlu olufunwayo lwe-pH kwiintlobo ezahlukeneyo zezicelo.I-TRIS-Acetate iluncedo kakhulu kwi-DNA kunye nezifundo ze-RNA, njengoko zibonelela. indawo efanelekileyo kwimisebenzi ye-enzyme, i-electrophoresis, kunye ne-gel electrophoresis.Inceda ukugcina ukuzinza kunye nokunyaniseka kwe-nucleic acids ngexesha leenkqubo ezahlukeneyo, ezifana ne-DNA sequencing, i-polymerase chain reaction (PCR), kunye ne-agarose gel electrophoresis.Ngaphezu kophando lwe-nucleic acid, i-TRIS-Acetate iphinda isetyenziswe kwiiprotheni zodwa kunye neenkqubo zokucoca. , i-membrane protein extraction, kunye novavanyo lwenkcubeko yeseli.Umthamo wayo oguquguqukayo webuffering uwenza ube sisixhobo esixabisekileyo kwiindawo ezininzi zophando lwezenzululwazi, iqinisekisa iimeko ezifanelekileyo zokusabela kwebhayoloji kunye nokugcina uzinzo lwee-enzymes kunye neeproteni.

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I-Tris-acetate (i-TRIS-Acetate) sisithintelo esisetyenziswa ngokuqhelekileyo kwimifuniselo yebhayoloji neyemichiza.Iqukethe indibaniselwano ye-tris(hydroxymethyl)aminomethane (Tris) kunye ne-acetic acid, esebenza njengomlawuli we-pH kunye nokuzinzisa.I-pH ye-TRIS-Acetate buffer ngokuqhelekileyo isuka kwi-7.4 ukuya kwi-8.4.
Esona siphumo siphambili se-TRIS-Acetate kukugcina i-pH ezinzileyo, ebaluleke kakhulu kwiimpendulo ezininzi zebhayoloji kunye nezakhemikhali.Isebenza njengesithinteli ngokunciphisa naluphi na utshintsho olubalulekileyo kwi-pH enokuthi yenzeke ngenxa yeeasidi ezongeziweyo okanye iziseko ngexesha leenkqubo zovavanyo.
I-TRIS-Acetate ifumana izicelo ezahlukeneyo kwibhayoloji yemolekyuli, i-biochemistry, kunye ne-biotechnology:
I-DNA kunye ne-RNA Electrophoresis: I-TRIS-Acetate isetyenziswa ngokuqhelekileyo njenge-buffer esebenzayo kwi-agarose kunye ne-polyacrylamide gel electrophoresis.Inika indawo ezinzileyo ye-pH ngexesha lokuhlukana kwe-DNA kunye ne-RNA amaqhekeza ngokusekelwe kubukhulu bawo.
Uhlalutyo lweprotheyini: I-TRIS-Acetate buffers isetyenziselwa i-protein electrophoresis, njenge-SDS-PAGE (i-Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis).Iqinisekisa ukuzinza kweprotheyini kunye nokuhlukana ngexesha lenkqubo.
Iimpendulo ze-Enzyme: I-TRIS-Acetate buffers isetyenziswa rhoqo kwiimvavanyo ze-enzyme kunye nezifundo.Ibonelela ngoluhlu lwe-pH olufanelekileyo kwiimpendulo ezahlukeneyo ze-enzymatic kwaye inceda ukugcina umsebenzi we-enzyme.
I-Cell and Tissue Culture: I-TRIS-Acetate buffers isetyenziswe kwimidiya yenkcubeko yeseli ukugcina i-pH efanelekileyo yokukhula kweeseli kunye nokwanda.Inceda ukugcina iimeko zephysiological ezifunekayo ekusebenzeni kweeseli.

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Iinkcukacha ezongezelelweyo:

Ukuqamba	C6H15NO5
Isivavanyi	99%
Imbonakalo	Umgubo omhlophe
Inombolo yeCAS.	6850-28-8
Ukupakisha	Incinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Ugcino	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	ISO.