I-4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Ngokukodwa, isetyenziswa kakhulu ekufumaneni umsebenzi we-beta-mannosidase. Xa i-4-nitrophenyl-alpha-D-mannopyranoside iqhekezwe yi-beta-mannosidase, ikhupha imveliso enombala otyheli ebizwa ngokuba yi-4-nitrophenol. Ubukhali bombala otyheli buhambelana ngokuthe ngqo nobungakanani bomsebenzi we-beta-mannosidase okhoyo kwisampuli. Uvavanyo olusebenzisa i-4-nitrophenyl-alpha-D-mannopyranoside ludla ngokusetyenziswa ukufunda i-enzyme kinetics, ukulinganisa amanqanaba omsebenzi we-enzyme, kunye nokuhlola ii-mutants okanye izithinteli ze-beta-mannosidase. Le khompawundi ibonelela ngendlela ekhawulezayo nelula yokulinganisa ngokobuninzi umsebenzi wale enzyme ithile.