I-TRIS-Acetate CAS: 6850-28-8 Ixabiso lomenzi

I-TRIS-Acetate, sisithambisi esisetyenziswa kakhulu kwiimvavanyo zebhayoloji kunye nezebhayoloji. Yindibaniselwano yesiseko seTris kunye ne-acetic acid, nto leyo ekhokelela kwisisombululo esizinzileyo se-pH esisetyenziselwa ukulawula nokugcina uluhlu lwe-pH olufunekayo kwiintlobo ngeentlobo zezicelo. I-TRIS-Acetate iluncedo kakhulu kwizifundo ze-DNA kunye ne-RNA, njengoko ibonelela ngendawo efanelekileyo yemisebenzi ye-enzyme, i-electrophoresis, kunye ne-gel electrophoresis. Inceda ukugcina uzinzo kunye nokuthembeka kwee-nucleic acids ngexesha leenkqubo ezahlukeneyo, ezinje ngokulandelelanisa i-DNA, i-polymerase chain reaction (PCR), kunye ne-agarose gel electrophoresis. Ukongeza kuphando lwe-nucleic acid, i-TRIS-Acetate ikwasetyenziswa kwiinkqubo zokwahlulwa kweproteni kunye nokucoca, ukukhupha i-membrane protein, kunye novavanyo lwenkcubeko yeseli. Amandla ayo okuguqula ajikeleze ayenza ibe sisixhobo esibalulekileyo kwiindawo ezininzi zophando lwesayensi, ukuqinisekisa iimeko ezifanelekileyo zempendulo yebhayoloji kunye nokugcina uzinzo lwee-enzymes kunye neeproteni.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

I-Tris-acetate (TRIS-Acetate) yi-buffer esetyenziswa kakhulu kwiimvavanyo zebhayoloji kunye nezebhayoloji. Iqulathe indibaniselwano ye-tris(hydroxymethyl)aminomethane (Tris) kunye ne-acetic acid, esebenza njengomlawuli we-pH kunye ne-stabilizer. I-pH ye-TRIS-Acetate buffer idla ngokuba phakathi kwe-7.4 ukuya kwi-8.4.
Isiphumo esiphambili se-TRIS-Acetate kukugcina i-pH ezinzileyo, ebalulekileyo kwiimpendulo ezininzi zebhayoloji kunye nezebhayoloji. Isebenza njenge-buffer ngokunciphisa naluphi na utshintsho olubalulekileyo kwi-pH olunokwenzeka ngenxa yee-asidi ezongezelelweyo okanye iziseko ngexesha leenkqubo zovavanyo.
I-TRIS-Acetate ifumana izicelo ezahlukeneyo kwi-molecular biology, i-biochemistry, kunye ne-biotechnology:
I-DNA kunye ne-RNA Electrophoresis: I-TRIS-Acetate isetyenziswa kakhulu njenge-buffer esebenzayo kwi-agarose kunye ne-polyacrylamide gel electrophoresis. Ibonelela ngendawo ye-pH ezinzileyo ngexesha lokwahlulwa kweziqwenga ze-DNA kunye ne-RNA ngokusekelwe kubukhulu bazo.
Uhlalutyo lweeProtein: Ii-buffers ze-TRIS-Acetate zisetyenziselwa i-protein electrophoresis, njenge-SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis). Iqinisekisa uzinzo kunye nokwahlukana kweeprotein ngexesha lenkqubo.
Iimpendulo ze-Enzyme: Ii-buffers ze-TRIS-Acetate zihlala zisetyenziswa kwiimvavanyo ze-enzyme kunye nezifundo. Ibonelela ngoluhlu lwe-pH olufanelekileyo kwiimpendulo ezahlukeneyo ze-enzyme kwaye inceda ukugcina umsebenzi we-enzyme.
Ukukhula kweeSeli kunye neeTishu: Ii-buffers ze-TRIS-Acetate zisetyenziswa kwimidiya yokukhula kweeseli ukugcina i-pH efanelekileyo yokukhula nokwanda kweeseli. Inceda ukugcina iimeko zomzimba ezifunekayo ukuze iiseli zikwazi ukuphila.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C6H15NO5
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	6850-28-8
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.