Tris(hydroxymethyl)nitromethane CAS:126-11-4

Isiphumo:

I-Buffering Capacity: I-Tris isebenza njenge-arhente esebenzayo yokuthintela ngenxa yokukwazi kwayo ukwamkela okanye ukunikela ngeeproton, igcina uluhlu lwe-pH oluzinzileyo kwizisombululo.Iqhele ukusetyenziswa njengenxalenye ephambili kwiinkqubo zesithinteli ukuzinzisa i-pH yeesampulu zebhayoloji kunye neempendulo.

Usetyenziso:

Ibhayoloji yeMolekyuli: I-Tris isetyenziswa ngokubanzi njenge-agent ephazamisayo kwiindlela ezahlukeneyo zebhayoloji ye-molekyuli, kubandakanywa i-DNA kunye ne-RNA yodwa, i-PCR, i-gel electrophoresis, kunye nokucocwa kweprotheni.Inceda ukugcina indawo ezinzileyo ye-pH, ivumela iimeko ezifanelekileyo zokusabela kwe-enzymatic kunye nokusebenzisana kweemolekyuli.

Inkcubeko yeSeli: I-Tris ihlala isetyenziswa kwimidiya yenkcubeko yeseli ukugcina i-pH engaguqukiyo kunye ne-osmotic balance, ukuxhasa ukukhula kweeseli okunempilo kunye nokusebenza.

I-Protein Chemistry: I-Tris isetyenziswa kwiimvavanyo ze-protein chemistry, ezifana ne-protein solubilization, i-protein stability assays, kunye nezifundo zokubopha i-protein-ligand.Inceda ukugcina uluhlu lwe-pH olufunwayo, ukuqinisekisa ukusongwa kweprotheyini efanelekileyo kunye nomsebenzi.

I-Enzymology: I-Tris iqeshwe kwiimvavanyo ezahlukeneyo ze-enzymatic ukwandisa iimeko ze-pH ezifunekayo kumsebenzi we-enzymatic.Iqinisekisa iziphumo ezinokwethenjelwa kunye nokuveliswa kwakhona, okwenza umlinganiselo ochanekileyo we-enzyme kinetics kunye nezifundo zokuvimbela.

Uvavanyo lwe-Biochemical: I-Tris isetyenziswa njengenxalenye kwiimvavanyo ezininzi ze-biochemical ngenxa yeempawu zayo zokuphazamisa.Igcina i-pH eqhubekayo ngexesha le-colorimetric, i-spectrophotometric, kunye ne-enzymatic assays, ukuphucula ukuchaneka kunye nokuthembeka kweziphumo.