I-Uridine,2′-deoxy-5-ethynyl- CAS:61135-33-9

I-5-ethynyl-2′-deoxyuridine (EdU) yi-nucleoside analog esetyenziswa kakhulu kwicandelo lebhayoloji yemolekyuli kunye nophando lwebhayoloji yeseli. Isetyenziswa rhoqo ukulebhelisha nokufumanisa i-DNA entsha eyenziweyo kwiiseli eziphilayo ngenkqubo eyaziwa ngokuba yi-"click chemistry." Le ndlela ivumela ukubonakala kunye nokulinganisa ukuphindaphinda kwe-DNA kunye nokwanda kweeseli. I-EdU ibe yindlela ethandwayo kwi-thymidine analog bromodeoxyuridine (BrdU) yendabuko ngenxa yokuhambelana kwayo ne-fluorescence microscopy kunye nokungabikho kwamanyathelo aqatha okuguqula i-DNA ngexesha lokufumanisa. Ukongeza, ulwakhiwo lweekhemikhali lwe-EdU luvumela ukufunyanwa okuthe ngqo nokusebenzayo, okwenza kube sisixhobo esibalulekileyo sokufunda ukwenziwa kwe-DNA kunye ne-dynamics yomjikelo weseli.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Uvavanyo lokuKhula kweeSeli: I-EdU inokufakwa kwi-DNA ephindaphindayo ngexesha lesigaba se-S somjikelo weseli. Oku kuyenza ibe sisixhobo esibalulekileyo sokufunda ukwanda kweeseli kunye nokwenziwa kwe-DNA.

I-Click Chemistry: I-EdU idla ngokusetyenziswa kunye ne-click chemistry, impendulo ekhethiweyo neyimveliso ephezulu esetyenziselwa ukufumanisa ii-biomolecules. Le ndlela ivumela ukufunyanwa kwe-DNA ebhalwe yi-EdU kwiiseli kunye nezicubu.

I-Fluorescence Microscopy: Ukufakwa kwe-EdU kuvumela ukubonakala kokwenziwa kwe-DNA phantsi kwe-fluorescence microscope. Le ndlela ibonelela ngesixhobo esibalulekileyo sokufunda ukwahlukana kweseli kunye nokwanda kwayo.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C11H12N2O5
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	61135-33-9
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.