I-2,3,5-Triphenyltetrazolium chloride CAS:298-96-4

I-2,3,5-Triphenyltetrazolium chloride, edla ngokubizwa ngokuba yi-TTC, isetyenziswa kakhulu njengesalathisi se-redox kwizicelo zebhayoloji kunye neekhemikhali. Enye yezicelo zayo eziphambili kukuvavanya ukusebenza kweseli kunye nomsebenzi we-metabolic. Xa i-TTC yongezwa kwiiseli eziphilayo okanye kwizicubu, inokuncitshiswa zii-enzymes ze-dehydrogenase kwi-mitochondria, nto leyo ekhokelela ekudalweni kwemveliso ye-formazan enombala obomvu.

Olu hlobo lokusabela ludla ngokusetyenziswa ukuvavanya ukuphefumla kweseli kunye nomsebenzi we-metabolism kwi-microbiology, i-cell biology, kunye nezifundo ze-plant physiology. Ubukhali bombala obomvu obubunjiweyo buhambelana nomsebenzi wee-enzymes ze-dehydrogenase kwaye bunokulinganiswa ngokwenani ukuvavanya ukusebenza kunye nomsebenzi we-metabolism weeseli okanye izicubu.

I-TTC ikwasetyenziswa njengophawu lokubakho kweeseli eziphilayo zeentsholongwane kunye nokuvavanya ukuba iintsholongwane zisengozini kangakanani kwiiarhente zokulwa neentsholongwane. Ukongeza, inezicelo kuvavanyo lomsebenzi weentsholongwane zomhlaba nakwiinkalo ezahlukeneyo zophando apho ukulinganisa umsebenzi we-metabolism yeseli kubalulekile.