3,3 ,5,5 -Tetramethylbenzidine dihydrochloride hydrate CAS:207738-08-7

I-3,3', 5,5'-Tetramethylbenzidine dihydrochloride (TMB) sisixhobo esisetyenziswa kakhulu kwi-enzyme-linked immunosorbent assays (ELISAs) nakwezinye ii-biochemical assays. Xa i-TMB i-oxidized yi-enzyme horseradish peroxidase (HRP) xa kukho i-hydrogen peroxide, ivelisa umbala oluhlaza okwesibhakabhaka. Olu tshintsho lombala lusetyenziselwa ukubona ubukho bee-antibodies ezibhalwe yi-HRP okanye ezinye iiproteni, ezihlala zibonisa ubukho be-antigen ethile kwisampulu. Umbala oluhlaza okwesibhakabhaka oveliswa yimpendulo uhlala ulinganiswa kusetyenziswa i-spectrophotometer, evumela ukulinganiswa kobunzulu botshintsho lombala kwaye inika indlela yokudibanisa ubunzulu bombala nobungakanani bemolekyuli ekujoliswe kuyo kwisampulu. I-TMB inezicelo ezininzi kwiimvavanyo zokuxilonga, uphando, kunye nophuhliso lwamayeza, ngakumbi ekufumaneni ii-antibodies, iihomoni, ii-cytokines, kunye nezinye ii-biomolecules. Uvakalelo lwayo, ukuzinza, kunye nokulula kokusetyenziswa kwayo kuyenza ibe sisixhobo esibalulekileyo kwiindawo ezahlukeneyo zelebhu zokufumanisa kunye nokulinganisa iimolekyuli ezithile.