3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS:3150-25-2

Ukufunyanwa komsebenzi we-beta-galactosidase: I-ONPG ihlala isetyenziselwa ukumisela ubukho kunye nomsebenzi we-beta-galactosidase kwiisampulu ezahlukeneyo zebhayoloji, ezinje ngeenkcubeko zebhaktiriya okanye iilysates zeseli.Ukuveliswa kwe-o-nitrophenol, onombala ophuzi, unokulinganiswa ngokulula usebenzisa i-spectrophotometer.

Izifundo zokubonisa iGene: I-ONPG iqhele ukusetyenziswa kuphando lwebhayoloji yemolekyuli ukufunda ukubonakaliswa kofuzo.Ngokudibanisa umgqugquzeli wemfuza yomdla kunye ne-gene encoding beta-galactosidase, abaphandi banokulinganisa umsebenzi walo mkhuthazi ngokongeza i-ONPG kunye nokulinganisa imveliso ye-o-nitrophenol.Le ndlela, eyaziwa ngokuba yi-beta-galactosidase reporter assay, inika ulwazi malunga nomsebenzi wokubhaliweyo we-gene.

Ukuchongwa kwebhaktheriya: Ezinye iibhaktheriya zivelisa i-beta-galactosidase, ngelixa ezinye zingenzi.I-ONPG inokusetyenziswa ngokudityaniswa nolunye uvavanyo lwe-biochemical ukuchonga iintlobo zebhaktiriya ngokusekwe kubuchule bazo bokwenza i-hydrolyze i-ONPG.Le ndlela isetyenziswa ngokuqhelekileyo kuxilongo lweklinikhi kunye nelebhu ze-microbiology.

Ukuhlolwa kwe-enzyme inhibitors okanye i-activators: I-ONPG ingasetyenziselwa ukukhuselwa kwiikhompawundi ezimodareyitha umsebenzi we-beta-galactosidase.Ngokulinganisa umsebenzi we-enzyme phambi kweekhompawundi ezahlukeneyo, abaphandi banokuchonga i-inhibitors okanye i-activators ezinokuthi ziphandwe ngakumbi ngenxa yamandla abo onyango.