I-4-Morpholineethanesulfonic acid CAS:4432-31-9

Ukuhluza i-pH: I-MES inexabiso le-pKa elimalunga ne-6.1, nto leyo eyenza ukuba ibe yi-buffer esebenzayo kuluhlu lwe-pH oluphakathi kwe-5.5 ukuya kwi-6.7. Inceda ukugcina i-pH izinzile ngokuxhathisa utshintsho kwi-asidi okanye i-alkalinity. Oku kuluncedo ngakumbi kwiimvavanyo kunye novavanyo olufuna indawo ethile ye-pH.

Izifundo ze-Enzyme: I-MES isetyenziswa rhoqo kuphando lwee-enzyme kunye novavanyo ngenxa yokuhambelana kwayo nee-enzymes ezahlukeneyo. Inceda ukugcina iimeko ze-pH ezifanelekileyo zomsebenzi wee-enzyme, ukuqinisekisa iziphumo ezichanekileyo nezithembekileyo.

Ukucocwa kweProtheyini: I-MES isetyenziswa kwiinkqubo zokucocwa kweprotheyini, ezifana ne-chromatography, ukugcina uzinzo kunye nomsebenzi weprotheyini ekujoliswe kuyo. Inceda ekugcineni ukwakheka kweprotheyini kunye nokusebenza kwayo ngexesha lokucoca.

I-Electrophoresis: I-MES isetyenziswa rhoqo kwiinkqubo ze-gel electrophoresis, ingakumbi ekuhlukaniseni nasekuhlalutyeni iiproteni ezincinci kunye neepeptides. Umthamo wayo wokubopha uqinisekisa i-pH ezinzileyo, ebalulekileyo ekuboniseni ngokuchanekileyo kunye nokuchazwa kweebhendi zeproteni.

Inkcubeko yeeseli: I-MES isetyenziswa rhoqo kwizifundo zenkcubeko yeeseli kunye ne-media formulations njenge-arhente yokukhusela. Inceda ukugcina i-pH ikwinqanaba elifanelekileyo lokukhula kweeseli, ukuphila, kunye neenkqubo ze-biochemical ngaphandle kokuphazamisa imisebenzi yeeseli.

Iimpendulo zeekhemikhali: I-MES ingasetyenziswa njenge-reagent kwiimpendulo zeekhemikhali kuba inokusebenza njengesiseko esibuthathaka okanye i-asidi. Amandla ayo okugcina i-bafa anceda ukugcina i-pH engaguqukiyo ngexesha lempendulo, okwenza kube lula ukulawula nokuphindaphinda ngcono.