I-4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS: 3459-18-5

I-Enzyme Substrate: i-pNAG isetyenziswa ngokubanzi njenge-substrate ethile yee-enzymes ezahlukeneyo ezibandakanyekayo kwi-hydrolysis ye-β-D-glucoside bond.Xa ezi enzymes ziqhekeza imolekyuli ye-pNAG, ikhupha i-p-nitrophenol.Oku kuvumela abaphandi ukuba balinganise kwaye balinganise umsebenzi we-enzymatic.

Uvavanyo lwe-Enzyme Activity: I-hydrolysis ye-pNAG ngama-enzymes athile inokubonwa kwaye ilinganiswe nge-spectrophotometrically.Oku kwenza i-pNAG ilungele ukuvavanya umsebenzi we-enzyme, apho inani le-p-nitrophenol eliveliswayo lihambelana ngokuthe ngqo nomsebenzi we-enzymatic.

Ukuhlolwa kwe-High-Throughput Screening: i-pNAG isetyenziswa ngokuqhelekileyo kwiimvavanyo zokuvavanya ukukhangela kunye nokuchonga i-enzyme inhibitors okanye i-activators.Ngokuphonononga impembelelo yeekhompawundi ezahlukeneyo kumsebenzi we-enzymatic, abaphandi banokuchonga abaviwa beziyobisi abanokubakho okanye iimodyuli zomsebenzi we-enzyme.

I-Gene Expression Studies: i-pNAG ikwasetyenziswa kuphando lwebhayoloji yemolekyuli ukufunda ukuchazwa kofuzo kunye nommiselo.Ngokulinganisa umsebenzi we-enzymatic we-enzymes ezithile usebenzisa i-pNAG njenge-substrate, abaphandi banokuphanda umphumo we-gene expression kumsebenzi we-enzyme kunye nomsebenzi.