I-TAPSO CAS: 68399-81-5 Ixabiso lomenzi

I-TAPSO (3-[N-tris(hydroxymethyl)methyl]amino]-2-hydroxypropanesulfonic acid) yi-zwitterionic buffer esetyenziswa kakhulu kuphando lwebhayoloji kunye ne-biochemical. Yi-arhente esebenzayo yokukhusela ene-pKa esondele kwi-pH ye-physiological, okwenza ifaneleke ukugcina i-pH ezinzileyo kwiimvavanyo zebhayoloji. I-TAPSO idla ngokusetyenziswa ekucoceni iiproteni, uvavanyo lwee-enzyme, inkcubeko yeeseli, kunye nokusetyenziswa kwe-electrophoresis. Ukunyibilika kwayo emanzini amaninzi kunye nokuphazamiseka okuncinci kwiinkqubo zebhayoloji kwenza ukuba ibe lukhetho oludumileyo kuluntu lwezenzululwazi. I-TAPSO yaziwa ngempembelelo yayo encinci kumsebenzi wee-enzyme kwaye idla ngokusetyenziswa njengendlela eyahlukileyo kwezinye ii-agent zokukhusela ezifana ne-Tris okanye ii-phosphate buffers.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukucocwa kweproteni: I-TAPSO isetyenziswa rhoqo njenge-buffer kwiindlela zokucoca iiproteni ezifana ne-ion exchange chromatography kunye ne-size exclusion chromatography. Umthamo wayo we-buffering unceda ukugcina i-pH efunekayo kuyo yonke inkqubo yokucoca, ukuqinisekisa uzinzo lweproteni.

Uvavanyo lwe-enzyme: I-TAPSO isetyenziswa kwiimvavanyo zomsebenzi we-enzyme ukubonelela ngendawo ye-pH ehambelanayo efana neemeko zomzimba. Ngokugcina i-pH ezinzileyo, i-TAPSO inceda ukuqinisekisa ukulinganiswa komsebenzi we-enzyme ngokuchanekileyo nangokuthembekileyo.

Inkcubeko yeeseli: I-TAPSO idla ngokusetyenziswa njenge-buffer yokugcina i-cell culture media kwi-pH engaguqukiyo. Indalo yayo ye-zwitterionic inciphisa ukusebenzisana neeseli kwaye inciphisa iziphumo ezinokubakho ze-cytotoxic ezinokuvela ngokusebenzisa ezinye ii-agents ze-buffering.

I-Electrophoresis: I-TAPSO ingasetyenziswa njenge-buffer esebenzayo kwiindlela ze-electrophoretic, ezifana ne-protein gel electrophoresis (SDS-PAGE) okanye i-capillary electrophoresis. Umthamo wayo wokubopha unceda ukugcina i-pH efunekayo ngexesha lenkqubo yokwahlulahlula.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C6H14NNaO4
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	68399-81-5
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.