I-Tapso Sodium CAS: 105140-25-8 Ixabiso lomenzi

Uzinzo lwe-pH: I-TAPS idla ngokusetyenziswa njenge-buffer yokuzinzisa i-pH yezisombululo kwiimvavanyo ezahlukeneyo kunye nokusetyenziswa. Ine-pKa ephantse ibe yi-pH ye-physiological, malunga ne-8.5, nto leyo eyenza ukuba ifaneleke ngakumbi ukugcina i-pH engaguqukiyo kwiinkqubo zebhayoloji.

Izifundo zeproteni: I-TAPS isetyenziswa kakhulu kwizifundo ze-biochemistry yeproteni kunye nebhayoloji yesakhiwo. Isetyenziswa kakhulu njenge-arhente yokuthintela izisombululo zeproteni kunye ne-buffers ukugcina ukwakheka kweproteni kunye nozinzo ngexesha lokucocwa, ukugcinwa, kunye novavanyo. I-TAPS iluncedo ngakumbi xa usebenza ngeeproteni ezinovelwano kutshintsho lwe-pH.

Uvavanyo lwe-enzyme: I-TAPS isetyenziswa rhoqo njenge-buffer kwi-enzymatic assays, apho i-pH kufuneka ilawulwe ngokuchanekileyo. Amandla ayo akhethekileyo okugcina i-buffer kunye nozinzo kuyenza ibe lukhetho olukhethwayo kwiintlobo ngeentlobo zeempendulo ze-enzyme.

Inkcubeko yeeseli: I-TAPS idla ngokubandakanywa kwi-cell culture media njenge-arhente yokukhusela ukugcina iimeko ze-pH ezifanelekileyo zokukhula kweeseli. Ukuhambelana kwayo kunye nozinzo kuyenza ibe luncedo kwiintlobo ngeentlobo zeeseli kuphando nakwizicelo zoshishino.

I-Electrophoresis: I-TAPS ingasetyenziswa njenge-arhente yokubopha kwiindlela ze-gel electrophoresis, ezifana ne-SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis). Inceda ukugcina i-pH ezinzileyo ngexesha lokwahlulwa kunye nohlalutyo lweeproteni, ii-nucleic acids, kunye nezinye ii-biomolecules.