Ityuwa ye-4-Nitrophenyl phosphate di(tris) CAS:68189-42-4

Ityuwa ye-4-Nitrophenyl phosphate di(tris) isetyenziswa kakhulu njengesiseko se-alkaline phosphatase kwiimvavanyo ezahlukeneyo ze-biochemical kunye ne-enzyme. Xa i-hydrolyzed yi-alkaline phosphatase, ivelisa imveliso enombala otyheli enokulinganiswa ngokulula nge-spectrophotometric. Le mpendulo isetyenziswa kakhulu kwizicelo ezifana ne-enzyme-linked immunosorbent assays (ELISAs) kunye nokufumanisa umsebenzi we-phosphatase.

Ubungakanani bombala otyheli obuveliswayo buhambelana ngqo nomsebenzi we-enzyme, nto leyo evumela ukuba kulinganiswe ngokuchanekileyo amanqanaba e-alkaline phosphatase kwiisampuli zebhayoloji. Oku kuyenza ibe luncedo kwiindawo zonyango nezophando ekufundeni umsebenzi we-alkaline phosphatase kwiiseli, kwizicubu, nakwii-biological fluids.

Ukongeza, ityuwa ye-4-Nitrophenyl phosphate di(tris) izinzile kwaye inyibilika kakhulu emanzini, nto leyo eyenza ukuba ifaneleke kwiimeko ezahlukeneyo zovavanyo. Uvakalelo lwayo oluphezulu kunye nokulula kokulinganisa kwenza ukuba ibe lukhetho oludumileyo njengesiseko sovavanyo lwee-enzyme, inceda ekuvavanyeni i-enzyme kinetics, umsebenzi we-enzyme, kunye nokufunyanwa kwamayeza.