5,5-Dithiobis(2-nitrobenzoic acid) CAS:69-78-3 Umfanekiso Obonakalayo

I-5,5-Dithiobis (i-asidi ye-nitrobenzoic eyi-2) I-CAS:69-78-3

I-5,5-Dithiobis (2-nitrobenzoic acid), ekwaziwa ngokuba yi-DTNB okanye i-Ellman's reagent, yikhemikhali esetyenziswa kakhulu kuphando lwe-biochemical kunye ne-pharmaceutical. Yipowder etyheli, ekristale enyibilikayo kakhulu emanzini nakwi-organic solvents.

I-DTNB isetyenziswa kakhulu ukulinganisa uxinaniso lwamaqela e-sulfhydryl (-SH) kwiiproteni kunye neepeptides. Le compound isabela kunye nee-thiols, nto leyo ebangela ukwenziwa kwe-disulfide exutyiweyo kwaye ikhuphe i-2-nitro-5-thiobenzoate, enombala otyheli onokulinganiswa nge-spectrophotometric. Ngokulinganisa ubunzulu bombala otyheli, kunokwenzeka ukuqikelela uxinaniso lwee-thiols kwisampuli. Le ndlela idla ngokusetyenziswa ukufunda ukusebenzisana kweeproteni neeproteni, ukugoba kweeproteni, kunye neempendulo ze-enzyme ezibandakanya amaqela e-thiol.

Ukongeza kwi-thiol quantification, i-DTNB isetyenzisiwe kwezinye iindlela zokusebenza, ezinje ngokuvavanya imeko ye-redox yeeseli, ukufunda uzinzo lwee-metal-sulfur compounds, kunye nokulinganisa umsebenzi wee-enzymes ezahlukeneyo.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ubungakanani beThiol: I-DTNB isetyenziswa rhoqo ukuvavanya uxinano lweethiols kwiisampuli zeproteni. Olu lwazi lubalulekile ekufundeni ukusebenzisana kweproteni neproteni, ukugoba kweproteni, kunye neempendulo ze-enzyme ezibandakanya amaqela e-thiol. Impendulo ye-colorimetric ye-DTNB ivumela ukulinganiswa kweethiols ngokusebenzisa umlinganiselo we-spectrophotometric.

Uvavanyo lweRedox State: I-DTNB isetyenziselwe ukuvavanya imeko ye-redox ngaphakathi kweeseli, izicubu, okanye iisampuli zebhayoloji. Ngokulinganisa utshintsho kuxinzelelo lwe-thiol, abaphandi banokuqonda ngoxinzelelo lwe-oxidative, umsebenzi we-antioxidant, kunye neendlela zokubonisa i-redox.

Uvavanyo lwe-Enzyme: Indlela engaqhelekanga esebenza ngayo i-DTNB ngee-thiols iyenza ibe sisixhobo esibalulekileyo sovavanyo lwe-enzyme. Ii-enzyme ezininzi, ezifana ne-glutathione-S-transferases, i-cystathionine-γ-lyase, kunye ne-thioredoxin reductase, zixhomekeke kwi-thiol kwaye zinokulinganiswa okanye zivanywe kusetyenziswa i-DTNB.

Uhlalutyo Lozinzo lweeMetal-Sulfur Compounds: I-DTNB isetyenziselwa ukuvavanya uzinzo kunye nokusebenza kwakhona kweemetal-sulfur complexes kunye neemetalloproteins. Le compound inokubona kwaye ilinganise ubukho beethiols ezibandakanyekayo ekuqhekekeni kwee-ion zemetal, inika ulwazi olubalulekileyo malunga nokusebenzisana kwemetal-sulfur kunye nekhemistri yokulungelelanisa.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C14H8N2O8S2
Uvavanyo	99%
Inkangeleko	Iqinile elimthubi
Inombolo ye-CAS	69-78-3
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.