I-5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide ityuwa ye-cyclohexylammonium CAS:18656-96-7

Isiphumo se-5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt (X-glucuronide) kukukwazi kwayo ukuvelisa utshintsho oluluhlaza okwesibhakabhaka okanye umbala xa kukho i-enzyme beta-D-glucuronidase. Le mpendulo ye-enzyme yenzeka xa i-glycosidic bond phakathi kweqela le-indole kunye ne-glucuronic acid moiety iqhekeka yi-beta-D-glucuronidase.

Ukusetyenziswa kwe-X-glucuronide kukwizifundo zebhayoloji yemolekyuli kunye novavanyo lwe-enzyme. Isetyenziswa kakhulu njengesiseko sokufumanisa umsebenzi we-beta-D-glucuronidase. Ezinye zezicelo ezithile ziquka:

Uvavanyo lwe-Reporter gene: I-X-glucuronide idla ngokusetyenziswa njengesiseko kwi-reporter gene assays apho i-beta-D-glucuronidase isetyenziswa njenge-reporter enzyme. Ngokudibanisa ummandla we-promoter okanye ulandelelwano lwe-code ye-gene enomdla kwi-beta-D-glucuronidase gene, abaphandi banokulinganisa ukubonakaliswa okanye umsebenzi we-gene ngokujonga ukwakheka kwe-blue precipitate okanye utshintsho lombala xa i-X-glucuronide yongezwa.

Uvavanyo lokuguqulwa kwebhaktheriya kunye nemvubelo: I-X-glucuronide ingasetyenziselwa ukubona utshintsho oluphumelelayo lwebhaktheriya okanye imvubelo ngevector equlethe i-beta-D-glucuronidase gene. Iiseli eziguqulweyo eziveza i-enzyme zinokufaka i-hydrolyze i-X-glucuronide, zivelise utshintsho olubonakalayo lombala okanye ukuchacha.

Ukufaka imibala kwi-histochemical: I-X-glucuronide isetyenziswa kwiindlela zokufaka imibala kwi-histochemical ukuze kubonwe ubukho kunye nokusasazwa komsebenzi we-beta-D-glucuronidase kwizicubu okanye kwiiseli. Oku kufakela imibala kuvumela indawo kunye nokuchongwa kweeseli eziveza i-enzyme.