I-8-Anilino-1-naphthalenesulfonic acid CAS:82-76-8

I-8-Anilino-1-naphthalenesulfonic acid, ekwaziwa ngokuba yi-ANS, yidayi ekhanyayo esetyenziswa kakhulu kuphando lwe-biochemical kunye ne-biophysical. Ikwiklasi yeekhompawundi ze-organic ezaziwa ngokuba yi-aniline-based sulfonic acids. I-ANS yaziwa ngokukwazi kwayo ukubopha kwiindawo ezingabonakaliyo kwi-hydrophobic okanye kwiindawo ezingabonakaliyo kwi-hydrophobic kwiiproteni nakwezinye ii-biomolecules.

I-ANS iyakhanya xa ibotshelelwe kwiindawo ezingenanto, nto leyo eyenza ukuba ibe yiprobe eluncedo yokufunda utshintsho kwimo yeproteni, ukusebenzisana kweproteni neproteni, kunye neenkqubo zokugoba/ukuvuleka kweproteni. Ukubopha kwe-ANS kwiindawo ezingenanto kukhokelela kutshintsho oluhlaza okwesibhakabhaka kwi-emission spectrum, enokubekwa esweni kusetyenziswa i-fluorescence spectroscopy.

I-ANS idla ngokusetyenziswa kwiindlela ezahlukeneyo zophando, kubandakanya i-fluorescence polarization, i-fluorescence resonance energy transfer (FRET), kunye ne-thermal stability assays. Isetyenziselwe ukufunda ulwakhiwo kunye nozinzo lweeproteni, ukusebenzisana kweeproteni kunye ne-ligand, kunye neenkqubo zokuhlanganiswa kweeproteni.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Iprobe yeFluorescent: I-ANS isetyenziswa kakhulu njengeprobe yefluorescent yokufunda utshintsho kwiproteni, iinkqubo zokugoba/ukuvuleka kweproteni, kunye nokusebenzisana kweproteni ne-ligand. Ibopha kwiindawo ezingabonakaliyo kwi-hydrophobic okanye kwiindawo ezingabonakaliyo kwi-hydrophobic kwiiproteni, nto leyo ebangela ukuphuma kwe-fluorescence okunokubekwa esweni kusetyenziswa i-fluorescence spectroscopy.

Ukuchonga ukunganyangeki kwamanzi: I-ANS inovelwano kutshintsho olukwimeko yokunganyangeki kwamanzi kwindawo encinci. Ingasetyenziselwa ukufumanisa nokulinganisa ii-surfactants, iisepha, kunye nezinye izinto ezinganyangekiyo emanzini.

Uvavanyo lozinzo lweproteni: I-ANS isetyenziswa rhoqo kwiimvavanyo zozinzo lobushushu ukuvavanya uzinzo lweeproteni. Ngokujonga ubunzulu be-fluorescence yeeproteni ezibotshelelwe yi-ANS phantsi kwamaqondo obushushu ahlukeneyo, kunokwenzeka ukumisela iimpawu zokutshintsha kobushushu kunye nobushushu bokunyibilika (Tm) kweproteni.

Izifundo zokuhlanganiswa kweeproteni: I-ANS ingasetyenziselwa ukufunda iinkqubo zokuhlanganiswa kweeproteni, njengokwenziwa kwee-amyloid fibrils. Le dayi inovelwano kwiindawo ezingabonakaliyo kwi-hydrophobic kwii-protein aggregates, nto leyo eyenza ukuba ibe sisixhobo esibalulekileyo sokujonga inkqubela phambili kunye ne-kinetics yokuhlanganiswa.

Ukuchazwa kwendlela ezisebenzisana ngayo iiproteni: I-ANS isetyenzisiwe ukuphanda indlela ezisebenzisana ngayo iiproteni. Xa iiproteni zidibana, kunokubakho utshintsho kwindlela ezisebenzisana ngayo emanzini, nto leyo enokubekwa esweni kusetyenziswa i-ANS fluorescence.

Ukufunyanwa nokuphuhliswa kwamayeza: I-ANS ingasetyenziswa ekuhlolweni kwamayeza anokuba ngamayeza. Inokunceda ekuvavanyeni ukuqina kokubopha kunye nokusebenzisana kweemolekyuli ezincinci neeproteni ezijoliswe kuzo, okuvumela abaphandi ukuba bachonge iikhompawundi ezinokuguqula ukusebenza kweproteni.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C16H13NO3S
Uvavanyo	99%
Inkangeleko	Umgubo oluhlaza obumnyama
Inombolo ye-CAS	82-76-8
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.