I-BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine ityuwa I-CAS:6578-06-9 Umfanekiso obonisiweyo

Ityuwa yeBCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine CAS:6578-06-9

I-BCIP-Toluidine, ekwaziwa ngokuba yityuwa ye-5-Bromo-4-chloro-3-indolylphosphate-p-toluidine, yikhemikhali esetyenziswa kakhulu kwi-molecular biology kunye nophando lwe-biochemistry. Isetyenziswa kakhulu njengesiseko kwi-enzyme-linked immunosorbent assays (ELISAs) okanye iindlela zokufaka idayi kwi-immunohistochemical ukuze kufunyanwe umsebenzi we-alkaline phosphatase.

Xa i-BCIP-Toluidine iqhekeka yi-alkaline phosphatase, ifumana utshintsho lombala, nto leyo evelisa i-blue-purple precipitate. Le mpendulo ivumela ukubonakala kunye nokulinganiswa komsebenzi we-alkaline phosphatase kwiisampuli. Ubungakanani bombala bunokulinganiswa nge-spectrophotometric kwaye buhambelana ngqo nomsebenzi we-enzyme.

Ukusetyenziswa kwe-BCIP-Toluidine njenge-substrate kuxhaphake kakhulu kwizicelo ezibandakanya ukulebheliswa kwe-DNA okanye i-RNA, njenge-in situ hybridization (ISH) okanye i-Southern and Northern blotting. Ibonelela ngendlela ethembekileyo neyomeleleyo yokufumanisa ubukho bee-nucleic acids ezijoliswe kuzo kwiisampuli.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

I-BCIP-Toluidine (ityuwa ye-5-Bromo-4-chloro-3-indolylphosphate-p-toluidine) yi-substrate esetyenziswa kwiindlela zebhayoloji ye-molecular ukufumanisa ubukho bomsebenzi we-alkaline phosphatase. Xa isetyenziswa njenge-substrate, i-BCIP-Toluidine ifumana impendulo yokutshintsha kombala xa iqhekeka yi-alkaline phosphatase, nto leyo ekhokelela ekudalweni kwe-blue-purple precipitate. Olu tshintsho lombala luvumela abaphandi ukuba babone ngamehlo kwaye balinganise umsebenzi we-alkaline phosphatase kwiintlobo ezahlukeneyo zovavanyo.

Eyona ndlela iphambili yokusebenzisa i-BCIP-Toluidine kukuvavanya i-immunosorbent assays (ELISAs) kunye neendlela zokufaka idayi kwi-immunohistochemical. Ihlala isetyenziselwa ukubona ii-antibodies ezidityaniswe ne-alkaline phosphatase okanye ii-probes ezibhalwe kwi-alkaline phosphatase kwezi vavanyo. I-BCIP-Toluidine isetyenziswa kakhulu kuphando lwebhayoloji yemolekyuli ukuze ibone kwaye ilinganise ukubonakaliswa kwezakhi zofuzo, indawo yeproteni, kunye nomsebenzi we-enzyme.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C8H6BrClNO4P.C7H9N
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	6578-06-9
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.