I-Bis[2-Hydroxyethyl] imino Tris-(Hydroxymethyl)-methane CAS:6976-37-0

I-Agent ye-Buffering: I-Bicine isebenza njenge-agent ye-buffering kwaye inceda ukugcina i-pH ezinzileyo kwizisombululo zamanzi. Inoluhlu olusebenzayo lwe-pH 7.6 ukuya kwi-9.0, nto leyo eyenza ukuba ibe luncedo kwizicelo ze-biochemical kunye ne-biological.

Uvavanyo lwe-Enzyme: I-Bicine isetyenziswa kakhulu kwiimvavanyo ze-enzyme kunye novavanyo lwe-biochemical ngenxa yokuhambelana kwayo neempendulo ze-enzyme. Inceda ukugcina i-pH efanelekileyo yomsebenzi we-enzyme, ivumela ukulinganiswa ngokuchanekileyo kwe-kinetics ye-enzyme kunye nomsebenzi.

I-Self Culture Media: I-Bicine isetyenziswa kwi-cell culture media njengomlawuli we-pH ukugcina iimeko zokukhula ezifanelekileyo kwiintlobo ezahlukeneyo zeeseli. Ibonelela ngendawo ezinzileyo yokukhula kweeseli kwaye iqinisekisa ukuba i-pH ihlala ngaphakathi koluhlu olufunekayo.

Ukucocwa kweeProteni: I-Bicine isetyenziswa kwiinkqubo zokucocwa kweeproteni, ingakumbi ngexesha le-ion exchange chromatography. Inceda ekususeni iiproteni kwaye inika amandla okugcina uzinzo lweproteni ecociweyo.

I-Electrophoresis: I-Bicine isetyenziswa kakhulu njenge-arhente yokubopha kwiindlela ze-gel electrophoresis, ezifana ne-polyacrylamide gel electrophoresis (PAGE). Inceda ukugcina i-pH ezinzileyo kwi-gel, ebaluleke kakhulu ekuhlukaniseni nasekuhlalutyeni iiproteni kunye nee-nucleic acids.

Iifomyula zamayeza: I-Bicine isetyenziswa ekwenzeni iimveliso zamayeza ukulungisa nokuzinzisa i-pH yesisombululo. Inokufumaneka kumayeza ahlukeneyo, kuquka iifomyula zolwelo, ii-injection, kunye nokulungiselela okungaphandle.