I-CHES Na CAS:103-47-9 Ixabiso lomvelisi

I-2-(i-Cyclohexylamino) i-ethanesulfonic acid yi-chemical compound kunye ne-molecular formula C10H21NO3S.Ikwaziwa ngesishunqulelo sayo esithi CHES.I-CHES yi-sulfonic acid derivative equkethe zombini iqela le-amino kunye neqela le-sulfonic acid kwisakhiwo sayo.

I-CHES iqhele ukusetyenziswa njenge-agent ephazamisayo kuphando lwebhayoloji kunye nebhayoloji.I-pH-stable kwaye igcina i-pH engaguquguqukiyo imeko-bume, ngakumbi kwiindawo zaselabhoratri ezibandakanya ukusabela kwe-enzymatic okanye izifundo zeprotheyini.I-CHES ine-pKa ye-9.3, iyenza ibe sisithinteli esisebenzayo malunga ne-pH 9.

Ubume bayo beekhemikhali ezizodwa kunye neepropathi zenza ukuba i-CHES ibe luncedo kwizicelo ezahlukeneyo, ezinje ngokulungiselela izisombululo ze-buffer ze-electrophoresis, i-enzyme assays, kunye nemidiya yenkcubeko yeeseli.Idla ngokukhethwa kwizicelo ezifuna uluhlu lwe-pH lwe-8.5 ukuya kwi-10.

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Isicelo kunye nesiphumo

Buffering: I-CHES iqhele ukusetyenziswa njenge-arhente ekhuselayo ukugcina i-pH ezinzileyo kwimifuniselo yebhayoloji kunye neempendulo ze-enzymatic.Ibaluleke kakhulu kwizicelo ezifuna uluhlu lwe-pH lwe-8.5 ukuya kwi-10.

I-Electrophoresis: I-CHES isetyenziswa rhoqo njenge-buffer kwiindlela ze-electrophoresis, ezifana ne-SDS-PAGE (i-Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis), ukwahlula iiprotheni ezisekelwe kubunzima be-molecular.

Uvavanyo lwe-Enzyme: I-CHES isetyenziswa kwiimvavanyo ze-enzyme ukugcina i-pH efanelekileyo yomsebenzi we-enzyme.Kuyanceda ukuqinisekisa ukuba ukusabela kwe-enzymatic kwenzeka phantsi kweemeko ezilawulwayo nezithembekileyo.

Imidiya yenkcubeko yeeseli: I-CHES ngamanye amaxesha ibandakanyiwe kwimidiya yenkcubeko yeeseli kwiindidi zeeseli ezahlukeneyo njengecandelo elilawula i-pH.Inceda ukugcina amanqanaba e-pH ye-physiological, ebaluleke kakhulu ekukhuleni kweeseli ezifanelekileyo kunye nokusebenza.

Izifundo zeprotheyini: I-CHES isoloko isetyenziswa ekucoceni iiprotheyini kunye novavanyo lwecrystallization yeprotheyini.Iimpawu zayo zokukhusela zinceda ukugcina uzinzo kunye nokunyaniseka kweeprotheni ngexesha lezi nkqubo.

Ukupakishwa kweMveliso:

Iinkcukacha ezongezelelweyo:

Ukuqamba	C8H17NO3S
Isivavanyi	99%
Imbonakalo	Umgubo omhlophe
Inombolo yeCAS.	103-47-9
Ukupakisha	Incinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Ugcino	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	ISO.