I-D-Luciferin CAS:2591-17-5 Ixabiso lomenzi

I-D-Luciferin yimolekyuli encinci ekhupha ukukhanya efumaneka kakhulu kwizinto eziphilayo ezikhanyayo ezifana neempukane, iibhaktheriya, kunye nezinto eziphilayo zaselwandle. Yinto ephambili kwimpendulo ye-luciferase, inkqubo ye-biochemical evelisa ukukhanya.

I-D-Luciferin idla ngokusetyenziswa njengesiseko sovavanyo lwe-bioluminescence kunye neendlela zokwenza imifanekiso, apho impendulo yayo kunye ne-luciferase ivelisa ukukhanya okunokufunyanwa nokulinganiswa. Oku kuyenza ibe sisixhobo esibalulekileyo kwiindawo ezahlukeneyo zophando, kubandakanya ibhayoloji yemolekyuli, ukufunyanwa kwamayeza, kunye nemifanekiso ye-biomedical.

Ngenxa yeempawu zayo ezizodwa zokukhanya, i-D-Luciferin isetyenziswe kakhulu kwizifundo ezibandakanya ukubonakaliswa kwezakhi zofuzo, ukusebenzisana kweproteni neproteni, kunye nokuhanjiswa kwemiqondiso kwiiseli. Ibonelela ngendlela engangenisi ntsholongwane neyobuthathaka yokuphanda iinkqubo zebhayoloji ngexesha langempela.

Ukongeza, kuye kwaveliswa ii-derivatives ze-D-Luciferin kunye nee-analogs, ezivumela utshintsho kunye nokusetyenziswa okuhlukeneyo. Ezi derivatives eziguquliweyo zivumela amaza ahlukeneyo okukhupha ukukhanya, uzinzo oluphuculweyo, kunye nokungena ngcono kwezicubu.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Imifanekiso ye-Bioluminescence: I-D-Luciferin isetyenziswa kakhulu kwiindlela zokwenza imifanekiso ye-bioluminescence, ezifana novavanyo lwe-luciferase reporter. Ngokungenisa ii-luciferase genes kwiiseli okanye kwizinto eziphilayo ezinomdla, umsebenzi okanye ukubonakaliswa kwee-genes ezithile kunokubonwa kwaye kulinganiswe. Le teknoloji ivumela abaphandi ukuba balandele kwaye bajonge iinkqubo zeseli ngexesha langempela.

Ukufunyanwa nokuphuhliswa kwamayeza: Uvavanyo lwe-bioluminescence olusekelwe kwi-D-Luciferin luyisixhobo esibalulekileyo ekufumaneni amayeza. Ngokufaka ii-genes ze-luciferase reporter kwiiseli okanye kwizinto eziphilayo ekujoliswe kuzo, abaphandi banokulinganisa impembelelo yamayeza okanye iikhompawundi zeekhemikhali kwiinkqubo ezithile zebhayoloji. Oku kwenza ukuba kuchongwe abantu abanokuba ngamayeza kunye novavanyo lokusebenza kwawo.

Uphando lwebhayoloji yeemolekyuli: I-D-Luciferin isetyenziswa kakhulu kwiindlela ezahlukeneyo zebhayoloji yeemolekyuli, ezinje ngokufunda ukusebenzisana kweproteni neproteni, ukujonga ukubonakaliswa kwezakhi zofuzo, kunye nokuphonononga iindlela zokubonisana kweeseli. Ukukwazi ukulinganisa ukukhutshwa kokukhanya ngobuninzi kubonelela ngendlela engangenisi ntsholongwane neyobuthathaka yokuphanda ezi nkqubo zebhayoloji.

Imifanekiso ye-in vivo: I-D-Luciferin ingasetyenziselwa i-in vivo imaging kwiimodeli zezilwanyana. Ngokufaka i-D-Luciferin kwisilwanyana, iiseli okanye izicubu eziveza i-luciferase zinokubonwa kwaye zijongwe kusetyenziswa iinkqubo ze-bioluminescence imaging. Oku kuvumela abaphandi ukuba bafunde inkqubela phambili yesifo, ukufuduka kweeseli, kunye neempendulo zonyango kwizilwanyana eziphilayo.

Ukubeka iliso kwindalo esingqongileyo: Iinkqubo ezisekwe kwi-D-Luciferin zisetyenzisiwe ekubekeni iliso kwindalo esingqongileyo, njengokufumanisa ungcoliseko lweentsholongwane ekutyeni nasemanzini. Ukubonakaliswa kwejini yeLuciferase kwiibhaktheriya kunokusetyenziswa ukuvavanya ukuphila kwazo kunye nomsebenzi wazo, okubonelela ngendlela ekhawulezayo neyobuchule yokubeka iliso kwiintsholongwane ezikwindalo esingqongileyo.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C11H8N2O3S2
Uvavanyo	99%
Inkangeleko	Umgubo otyheli okhanyayo
Inombolo ye-CAS	2591-17-5
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.