I-DDT CAS: 3483-12-3 Ixabiso lomvelisi

I-DL-Dithiothreitol, eyaziwa ngokuba yi-DTT, i-arhente yokunciphisa esetyenziswa ngokuqhelekileyo kuphando lwebhayoloji kunye ne-molecular biology.I-molecule encinci ene-thiol (equlethe isulfure) iqela kwinqanaba ngalinye.

I-DTT isetyenziswa rhoqo ukuphula iibhondi ze-disulfide kwiiprotheyini, ezinceda ukutyhila okanye ukudinwa.Oku kuncitshiswa kwamabhondi e-disulfide kubalulekile kwiinkqubo ezahlukeneyo zelabhoratri ezifana nokuhlanjululwa kweprotheyini, i-gel electrophoresis, kunye nezifundo zesakhiwo seprotheni.I-DTT ingasetyenziselwa ukukhusela amaqela e-thiol kunye nokuthintela i-oxidation ngexesha leenkqubo zovavanyo.

I-DTT yongezwa ngokuqhelekileyo kwizisombululo zokulinga kwiindawo ezincinci, kwaye umsebenzi wayo uxhomekeke kubukho be-oxygen.Kubalulekile ukuphatha i-DTT ngononophelo njengoko ivakalelwa emoyeni, ukushisa, kunye nokufuma, okunokunciphisa ukusebenza kwayo.

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Ukunciphisa i-Disulfide Bonds: I-DTT isetyenziselwa ukuphula iibhondi ze-disulfide, ezizibophelelo ezihlangeneyo ezenziwe phakathi kweentsalela ezimbini ze-cysteine kwiiprotheni.Ngokunciphisa ezi bhondi, i-DTT inceda iiprotheni ze-denature, ivumela ukufundwa kwesakhiwo kunye nomsebenzi wabo.

I-Protein Folding: I-DTT inokuncedisa ukugoqa iiprotheyini ezifanelekileyo ngokuthintela ukubunjwa kwebhondi ye-disulfide engalunganga.Inciphisa naziphi na iibhondi ze-disulfide ezingezizo ezendalo ezinokuthi zenze ngexesha lokusonga iprotein, ivumela iprotein ukuba yamkele ukuhambelana kwayo kwemveli.

Umsebenzi we-Enzyme: I-DTT inokusebenzisa i-enzymes ethile ngokunciphisa nayiphi na inhibitory bond disulfide ekhoyo.Ukongezelela, i-DTT inokuthintela i-oxidation yeentsalela ezibalulekileyo ze-cysteine, ezinokuthi ziyimfuneko kumsebenzi we-enzyme.

Ukuveliswa kwe-Antibody: I-DTT yongezwa ngokuqhelekileyo ukunciphisa izibophelelo ze-disulfide ngexesha lokuveliswa kwee-antibodies.Inceda ukuthintela ukubunjwa kweebhondi ze-disulfide ezingalunganga, ezinokuthintela ukubopha okufanelekileyo kwe-antigen.

Ukuzinzisa iiProtheyini: I-DTT ingasetyenziselwa ukuzinzisa iiprotheni ngokuthintela i-oxidation okanye ukuhlanganiswa kwazo.Inceda ukugcina imeko encitshisiweyo yeeprotheni ngexesha lokugcinwa kunye neenkqubo zovavanyo.

Ukunciphisa ii-Agents kwi-Biology ye-Molecular: I-DTT isoloko isetyenziswa kwiindlela ezahlukeneyo zebhayoloji ye-molekyuli ezifana nokulandelelana kwe-DNA, i-PCR, kunye nokuhlanjululwa kweeprotheyini.Inokunceda ukugcina imeko encitshisiweyo yamacandelo abalulekileyo, iqinisekisa iziphumo ezilungileyo zovavanyo.

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Iinkcukacha ezongezelelweyo:

Ukuqamba	C4H10O2S2
Isivavanyi	99%
Imbonakalo	Umgubo omhlophe
Inombolo yeCAS.	3483-12-3
Ukupakisha	Incinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Ugcino	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	ISO.