I-DNase I, evela kwiBovine Pancreas CAS:9003-98-9

I-DNase I, ephuma kwi-pancreas yenkomo, yi-enzyme eqhekeza ngokukodwa iimolekyuli ze-DNA ngokufaka i-hydrolyzing kwi-phosphodiester bonds kumqolo we-DNA. Isiphumo esiphambili se-DNase I kukuwohloka nokuqhekeka kwe-DNA.

Eyona ndlela iphambili yokusebenzisa i-DNase I kuphando lwebhayoloji yemolekyuli. Isetyenziswa kakhulu kwiimvavanyo ezahlukeneyo zokwahlula nokucoca i-DNA, ukususa ungcoliseko lwe-DNA ye-genomic kwiisampuli ze-RNA, kunye nokulawula i-DNA. I-DNase I isebenza ngokukodwa ekonakaliseni i-DNA kwii-protein-DNA complexes, ivumela abaphandi ukuba bafunde ukusebenzisana kwe-protein-DNA okanye basuse iiproteni ezibotshelelwe yi-DNA.

Ukongeza, i-DNase I isetyenziswa rhoqo kwiimvavanyo ze-DNA footprinting, ezinceda ekuchongeni iindawo ze-DNA ezibotshelelwe ziiproteni ezithile. Ngokunyanga isampuli ye-DNA nge-DNase I kwaye emva koko uhlalutye iziqwenga ze-DNA eziphumayo, abaphandi banokufumanisa iindawo ezibotshelelweyo zeeproteni ezibotshelelweyo ze-DNA.

I-DNase I ikwasetyenziswa kuhlalutyo lwe-chromatin, apho inceda ekugayweni kwe-chromatin (i-DNA kunye neeproteni ezinxulumene nayo) ukuze ifunde ulwakhiwo lwayo kunye nokulungelelaniswa kwayo. Ngokugaywa kwe-chromatin nge-DNase I, abaphandi banokuchonga iindawo ezifikelelekayo nezinobuthathaka ekuqhekekeni, bebonelela ngolwazi malunga nokulawulwa kwezakhi zofuzo kunye nolwakhiwo lwe-chromatin.

Ngaphezu koko, i-DNase I inezicelo zokuxilonga kwezonyango, ngakumbi ekufumaneni nasekulinganiseni i-DNA engenaseli egazini elijikelezayo. Ngokusebenzisa i-DNase I ukugaya i-DNA engajikeleziyo, kunokwenzeka ukuhlalutya nokufumanisa ulandelelwano oluthile lwe-DNA oluhambelana nezifo okanye ukungaqheleki kwemfuza.