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I-N, N-Bis (2-hydroxyethyl) -2-aminoethanesulfonic acid ityuwa yesodium CAS: 66992-27-6
I-N,N-Bis(2-hydroxyethyl) -2-aminoethanesulfonic acid ityuwa yesodium, ekwaziwa ngokuba yi-HEPES ityuwa yesodium, yikhompawundi yekhemikhali esetyenziswa ngokuqhelekileyo njenge-agent ephazamisayo ye-pH kwiilebhu zebhayoloji kunye neekhemikhali.Inceda ukugcina uluhlu lwe-pH oluzinzile, okwenza lube lulungele izicelo ezifana nenkcubeko yeeseli, i-enzyme assays, izifundo zeprotheyini, i-electrophoresis, kunye ne-pharmaceutical formulations.I-HEPES ityuwa yesodium iqinisekisa iimeko ezifanelekileyo kwiinkqubo zebhayoloji kwaye iphakamisa ukuchaneka nokuthembeka kweziphumo zovavanyo.
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I-S-Butyrylthiocholine iodide CAS: 1866-16-6
I-iodide ye-S-Butyrylthiocholine yikhompawundi yemichiza esetyenziswa ngokuqhelekileyo kwiimvavanyo ze-biochemical kunye ne-enzymatic.I-substrate ye-enzyme i-butyrylcholinesterase (BChE) kwaye isetyenziselwa ukulinganisa umsebenzi wayo.
Xa i-iodide ye-S-Butyrylthiocholine i-hydrolyzed yi-BChE, ivelisa i-thiocholine kunye ne-butyric acid njengemveliso.Ukukhululwa kwe-thiocholine kunokulinganiswa usebenzisa i-spectrophotometric okanye i-fluorometric assay, evumela ubungakanani bomsebenzi we-BChE.
I-iodide ye-S-Butyrylthiocholine isoloko isetyenziselwa izicwangciso zeklinikhi kunye nophando ukuvavanya umsebenzi we-BChE kwiisampuli ezifana ne-plasma yegazi okanye izicubu.Ingasetyenziselwa ukuvavanya umsebenzi we-enzymatic we-BChE kunye nendima yayo enokubakho kwiinkqubo ezahlukeneyo zebhayoloji, kunye nokuxilongwa kunye nokubeka iliso kwiimeko ezithile zonyango.
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I-ABTS (2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) ityuwa yediammonium) CAS:30931-67-0
I-Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), edla ngokubizwa ngokuba yi-ABTS, isetyenziswa ngokuqhelekileyo i-chromogenic substrate kwiimvavanyo ze-biochemical, ngokukodwa kwintsimi ye-enzymology.Yikhompawundi yokwenziwa esetyenziselwa ukulinganisa umsebenzi wee-enzymes ezahlukeneyo, kubandakanywa i-peroxidases kunye ne-oxidases.
I-ABTS ayinambala ngokwemo yayo ye-oxidized kodwa ijike ibe bluhlaza-luhlaza xa ifakwe i-enzayimu kwi-hydrogen peroxide okanye i-oxygen yemolekyuli.Olu tshintsho lombala lubangelwa ukubunjwa kwe-cation radical, ethatha ukukhanya kwi-spectrum ebonakalayo.
Ukusabela phakathi kwe-ABTS kunye ne-enzyme ivelisa imveliso enemibala enokulinganiswa nge-spectrophotometrically.Ubunzulu bombala buhambelana ngokuthe ngqo nomsebenzi we-enzymatic, okuvumela abaphandi ukuba bahlole ngokobungakanani be-enzyme kinetics, inhibition ye-enzyme, okanye i-enzyme-substrate interactions.
I-ABTS inoluhlu olubanzi lwezicelo kwiinkalo ezahlukeneyo, kubandakanya uxilongo lwezonyango, uphando lwamayeza, kunye nesayensi yokutya.Inobuntununtunu kwaye inika uluhlu olubanzi oluguquguqukayo, iyenza ibe lukhetho oludumileyo kwiimvavanyo ezininzi ze-biochemical.
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4-NITROPHENYLΑ-D-MALTOHEXAOSIDE CAS:74173-30-1
I-4-Nitrophenyl α-D-maltohexaoside yi-compound yeklasi ye-α-glycosidic linkages.Iphuma kwimaltose, eyi-disaccharide eyenziwe ngeeyunithi ezimbini zeglucose.Kule khompawundi, iqela le-hydroxyl yeyunithi yokuqala ye-glucose ifakwe endaweni ye-nitrophenyl moiety.
Le khompawundi idla ngokusetyenziswa njenge substrate kwiimvavanyo ze-enzymatic ukufunda umsebenzi wee-enzymes ezahlukeneyo, ngakumbi ezo zibandakanyeka kwi-carbohydrate metabolism.Iqela le-nitrophenyl livumela ukufunyanwa lula kunye nobungakanani bempendulo ze-enzymatic ngokulinganisa ukufunxa okanye i-fluorescence yemveliso ecandiweyo.
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IIPIPE CAS: 5625-37-6 Ixabiso lomvelisi
I-PIPES (i-piperazine-1,4-bisethanesulfonic acid) yi-zwitterionic buffering ikhompawundi edla ngokusetyenziswa kuphando lwebhayoloji kunye ne-biochemical.Sisithinteli se-pH esisebenzayo esinomthamo ophezulu wokugcina iimeko ze-pH ezizinzileyo kuluhlu lwe-pH lwe-6.1 ukuya kwi-7.5.I-PIPES inokuphazamiseka okuncinci kunye ne-biomolecules kwaye ifanelekile kwiimvavanyo ezixhomekeke kwiqondo lokushisa.Ihlala isetyenziselwa ubuchule be-gel electrophoresis kunye nokuqulunqwa kwamachiza njenge-agent agent.Ngokubanzi, i-PIPES yikhompawundi eguquguqukayo kwaye isetyenziswa ngokubanzi kwiseto ezahlukeneyo zovavanyo.
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3,3′,5,5′-Tetramethylbenzidine CAS:207738-08-7
I-3,3',5,5'-Tetramethylbenzidine, eyaziwa ngokuba yi-TMB, i-chemical compound isetyenziswa ngokuqhelekileyo njenge-chromogenic substrate kwi-enzyme-linked immunosorbent assays (ELISA) kunye nezinye iimvavanyo ze-biochemical.Ihlala isetyenziselwa ukufumanisa kunye nokulinganisa ubukho bee-enzymes ezifana ne-horseradish peroxidase (HRP) kwiisampuli zebhayoloji ezahlukeneyo.I-TMB ifumana utshintsho lombala ukusuka kokungenambala ukuya koluhlaza okwesibhakabhaka xa kukho ezi enzymes.Emva koko, impendulo inokuyekwa ngokudibanisa iasidi eguqula umbala ozuba ube ngumbala omthubi wokugqibela.Ubunzulu bombala otyheli bulingana nobungakanani be-enzyme ekhoyo, evumela ubungakanani.
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I-APS-5 CAS: 193884-53-6 Ixabiso lomvelisi
(4-Chlorophenyl) i-thio-methanol 1- (i-dihydrogen phosphate) ityuwa ye-disodium (1: 2) i-chemical compound eyeyeklasi ye-acridine derivatives.Iqukethe inkqubo ye-ring ye-10-methylacridine kunye neqela le-thioether elifakwe kwindawo ye-4-chlorophenyl.Ikhompawundi ikwaneqela le-methanol kunye namaqela amabini e-phosphate athatyathwe ngokuyinxenye yi-sodium ion.
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5-Bromo-4-chloro-3-indolyl phosphate disodium ityuwa CAS:102185-33-1
I-5-Bromo-4-chloro-3-indolyl phosphate disodium ityuwa (BCIP) yingxube yekhemikhali esetyenziswa ngokuqhelekileyo kwi-biology ye-molecular kunye ne-biochemistry applications.Yi-chromogenic substrate ye-alkaline phosphatase enzymes.
I-BCIP isoloko isetyenziswa ngokubambisana ne-nitroblue tetrazolium (NBT) njenge-substrate yokubona umsebenzi we-alkaline phosphatase.Xa i-BCIP i-dephosphorylated yi-alkaline phosphatase, iifom ze-blue precipitate, ezivumela ukubonwa kobukho okanye umsebenzi we-enzyme.
Le khompawundi iluncedo kakhulu kwizicelo ezifana ne-immunohistochemistry, in situ hybridization, kunye ne-enzyme-linked immunosorbent assays (ELISAs) ukufumanisa ubukho okanye indawo ye-biomolecules ethile okanye i-nucleic acids.I-precipitate eluhlaza okwesibhakabhaka eyenziwe yi-BCIP inika uphawu olubonakalayo olunceda ekuchongeni nasekuhlalutyeni iamolekyu ekujoliswe kuzo kwiisampuli zokulinga.
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Ityuwa yetyuwa yesodium CAS: 139-41-3 Ixabiso lomvelisi
I-N, N-Bis (2-hydroxyethyl) ityuwa ye-sodium ye-glycine yikhompawundi yemichiza esetyenziswa njenge-agent ephazamisayo kwizicelo ezahlukeneyo ze-biochemical kunye ne-biophysical.Inceda ukugcina izinga le-pH elizinzile kwiimeko zovavanyo, okwenza kube luncedo kwizifundo ze-enzyme, uphando lweprotheyini, inkcubeko yeseli, kunye neendlela zokuqhawula zaseNtshona.
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I-4-Aminophthalhydrazide AMPPD CAS: 3682-14-2
I-4-Aminophthalhydrazide, eyaziwa ngokuba yi-4-APhH, i-chemical compound kunye ne-molecular formula C8H8N2O.Iphuma kwiklasi ye-hydrazide compounds kwaye ivela kwi-phthalic acid.
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N-Acetyl-L-cysteine CAS: 616-91-1
I-N-Acetyl-L-cysteine (NAC) yindlela elungisiweyo ye-amino acid cysteine.Inika umthombo we-cysteine kwaye inokuguqulwa ngokulula kwi-tripeptide glutathione, i-antioxidant enamandla emzimbeni.I-NAC yaziwa ngepropathi yayo ye-antioxidant kunye ne-mucolytic, iyenza ibe luncedo kwizicelo ezahlukeneyo zempilo.
Njenge-antioxidant, i-NAC inceda ukukhusela iiseli kumonakalo obangelwa yi-radicals yamahhala, iintlobo ze-oksijini ezisebenzayo, kunye ne-toxins.Ikwaxhasa i-glutathione synthesis, edlala indima ebalulekileyo kwiinkqubo zokukhupha umzimba kunye nokugcina amajoni omzimba asempilweni.
I-NAC ifundwe ngezibonelelo zayo ezinokuthi zibekho kwimpilo yokuphefumla, ngakumbi kubantu abaneemeko ezinjenge-bronchitis engapheliyo, i-COPD, kunye ne-cystic fibrosis.Idla ngokusetyenziswa njenge-expectorant ukunceda ukubhitya kunye nokukhulula i-mucus, okwenza kube lula ukucima iindlela zomoya.
Ngaphezu koko, i-NAC ibonise isithembiso ekuxhaseni impilo yesibindi ngokuncedisa ekususweni kwezinto ezinobuthi, ezifana ne-acetaminophen, i-painkiller yentlungu eqhelekileyo.Isenokuba neziphumo ezikhuselayo kumonakalo wesibindi obangelwa kukusetyenziswa kotywala.
Ukongeza kwiipropathi zayo ze-antioxidant kunye nenkxaso yokuphefumla, i-NAC iye yaphononongwa ngezibonelelo zayo ezinokubakho kwimpilo yengqondo.Olunye uphando lubonisa ukuba lunokuba nefuthe elihle kwiingxaki zengqondo, ezifana nokudakumba kunye nokuphazamiseka okunyanzelekileyo (OCD).
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I-acetylthiocholine iodide CAS: 1866-15-5
I-acetylthiocholine iodide yi-chemical compound esetyenziswa ngokuqhelekileyo njenge-substrate kwii-assays ze-enzyme ukulinganisa umsebenzi we-acetylcholinesterase (AChE) ye-enzyme.I-AChE yi-enzyme eyenza i-hydrolyzes i-neurotransmitter acetylcholine, inyathelo elibalulekileyo ekupheliseni ukuhanjiswa komqondiso phakathi kweeseli ze-nerve.
Xa i-acetylthiocholine iodide isenziwa yi-AChE, iqela le-acetyl liyasuswa, okubangela ukubunjwa kwe-thiocholine kunye ne-acetate ions.I-Thiocholine emva koko isabela nge-reagent engenambala ebizwa ngokuba yi-DTNB (5,5′-dithiobis(2-nitrobenzoic acid)) ukuvelisa ikhompawundi enombala omthubi ebizwa ngokuba yi-5-thio-2-nitrobenzoate, enokulinganiswa nge-spectrophotometrically.Umlinganiselo wokuphuhliswa kombala uhambelana ngokuthe ngqo nomsebenzi we-AChE kwisampuli.
