Ityuwa ye-5-Bromo-4-chloro-3-indolyl phosphate disodium CAS:102185-33-1

I-Immunohistochemistry (IHC): I-BCIP idla ngokusetyenziswa kwiimvavanyo ze-IHC ukuze kubonwe ubukho okanye indawo yee-antigens ezithile kwiisampuli zezicubu. I-blue precipitate eveliswa yi-BCIP ivumela ukuchongwa kweendawo ze-antigenic kwaye ingasetyenziselwa uhlalutyo lomgangatho okanye oluyi-semi-quantitative.

I-In situ hybridization (ISH): I-BCIP isetyenziswa kakhulu kwiindlela ze-ISH ukufumanisa nokuqonda ulandelelwano oluthile lwe-nucleic acid, njenge-mRNA okanye i-DNA. I-blue precipitate eyenziwe yi-BCIP ibonisa ubukho be-target sequences kwaye inceda ekuboniseni ukusasazwa kwazo ngaphakathi kweeseli okanye kwizicubu.

Ii-enzyme-linked immunosorbent assays (ELISAs): I-BCIP ngamanye amaxesha isetyenziswa kwi-ELISA ukufumanisa ubukho okanye ukuxinana kwee-biomolecules ezithile, ezifana neeproteni okanye ii-antibodies. Ngokudibanisa i-BCIP nelebhile ye-enzyme efanelekileyo kunye nokusebenzisa i-alkaline phosphatase ukuvuselela impendulo, umbala oluhlaza okwesibhakabhaka oveliswayo unokubonisa ubukho kunye nobuninzi be-biomolecule enomdla.