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I-Egtazic acid CAS: 67-42-5 Ixabiso lomvelisi
I-Ethylenebis(oxyethylenenitrilo)tetraacetic acid (EGTA) yi-agent ye-chelating eqhele ukusetyenziswa kuphando lwebhayoloji kunye neekhemikhali.Yikhompawundi yokwenziwa ephuma kwi-ethylenediamine kunye ne-ethylene glycol.I-EGTA inobudlelwane obuphezulu bee-ion zensimbi ezidityanisiweyo, ngakumbi i-calcium, kwaye isetyenziswa ngokubanzi ukutshekisha kunye nokuhlutha ezi ion kwizicelo ezahlukeneyo ezifana nenkcubeko yeeseli, uvavanyo lwe-enzyme, kunye nobuchule bebhayoloji yemolekyuli.Ngokuzibophelela kwi-calcium kunye nezinye i-ion zetsimbi, i-EGTA inceda ukulawula ukugxininiswa kwabo, ngaloo ndlela ibe nefuthe kwiinkqubo ezahlukeneyo ze-biochemical.
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I-P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7
I-P-Nitrophenyl beta-D-lactopyranoside, ekwabizwa ngokuba yi-PNPG, yikhompawundi ehlala isetyenziswa kwiimvavanyo ze-enzymatic ukulinganisa umsebenzi we-beta-galactosidase, i-enzyme ebandakanyeka kwi-carbohydrate metabolism.I-PNPG yi-substrate yokwenziwa enokuthi iqhekezwe yi-beta-galactosidase, ekhokelela ekukhululweni kwemveliso enemibala ephuzi.Ubungakanani be-substrate hydrolysis bunokubalwa ngokwe-spectrophotometrically ngokulinganisa ukufunxwa kwemveliso kumgama wobude obuthile.Oku kuvumela abaphandi ukuba bahlole umsebenzi kunye ne-kinetics ye-beta-galactosidase kwiimeko ezahlukeneyo, ezifana nokufunda umsebenzi we-enzyme, ukuhlolwa kwe-enzyme inhibitors okanye i-activators, okanye ukuvavanya imiphumo yokuguqulwa komsebenzi we-enzyme.
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popso disodium CAS: 108321-07-9
I-Piperazine-N,N'-bis(2-hydroxypropanesulphonic acid) ityuwa ye-disodium yikhompawundi yemichiza eyenziwe ngamaqela e-piperazine, i-bis(2-hydroxypropanesulphonic acid), kunye ne-sodium ion ezimbini.Isetyenziswa ngokuqhelekileyo njenge-arhente ekhuselayo kunye nomlawuli we-pH kwizicelo ezahlukeneyo zemizi-mveliso nezaselabhu.I-compound inceda ukugcina i-pH ethile kwizisombululo, iyenza ibe luncedo kwiinkqubo ezifana nokuhlanjululwa kweprotheyini, i-biology ye-molecular, kunye nophando lwamachiza.Ukongeza, inokuphinda isebenze njengearhente ye-chelating yeeyoni zetsimbi kwaye izinzise umsebenzi we-enzyme kwiimpendulo ezithile ze-biochemical.
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4-(2-hydroxyethyl) piperazine-1-ethane-sulfon.ac.hemiso.S CAS:103404-87-1
I-4-(2-Hydroxyethyl) piperazine-1-ethanesulfonic acid hemisodium ityuwa, ekwaziwa ngokuba yiCAPSO Na, yikhompawundi yemichiza edla ngokusetyenziswa njenge-agent ephazamisayo kwizicelo zebhayoloji yebhayoloji kunye nemolekyuli.Yityuwa ye-zwitterionic enceda ukulawula nokugcina i-pH ezinzileyo ngaphakathi koluhlu oluthile.I-CAPSO Na iyahambelana neenkqubo zebhayoloji, iyenza ifaneleke kwiimvavanyo ezahlukeneyo ze-enzymatic, ubuchule bokucoca iiprotheyini, kunye nemidiya yenkcubeko yeseli.Ikwasetyenziswa kwiindlela ze-electrophoresis kwaye iyaziwa ngokuzinza kwayo kwe-pH kunye nokuhambelana ne-enzymes.
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IPHENYL-1-THIO-β-D-GALACTOPYRANOSIDE CAS:16758-34-2
I-PHENYL-1-THIO-β-D-GALACTOPYRANOSIDE, eyaziwa ngokuba yi-phenyl thio galactopyranoside, yikhemikhali yekhemikhali yentsapho ye-glycosides.I-derivative derivative ye-galactose equkethe iyunithi yeshukela ye-galactopyranose efakwe kwiqela le-phenylthio kwi-anomeric carbon.Le khompawundi isetyenziswa ngokuqhelekileyo kuphando lwe-biochemical njenge-substrate ye-enzymes eyenza i-hydrolyze glycosidic bonds.Isebenza njenge-artificial substrate ukufunda umsebenzi we-enzymatic we-glycosidases kunye nokumisela ukuchaneka kwabo, i-kinetics, kunye ne-inhibition.I-PHENYL-1-THIO-β-D-GALACTOPYRANOSIDE idla ngokusetyenziswa kwii-assays ze-colorimetric kunye ne-fluorometric ukubona ubukho okanye ukulinganisa umsebenzi wee-glycosidase ezahlukeneyo kwiisampulu zebhayoloji.I-hydrolysis yale ngqungquthela nge-enzymes ethile ivelisa uphawu olubonakalayo olunokuthi lubalwe.Ngenxa yeqela layo le-phenylthio elizinzile, i-PHENYL-1-THIO-β-D-GALACTOPYRANOSIDE inokuphathwa ngokulula kwaye igcinwe ngaphandle kokubola, okwenza kube yinto efanelekileyo yokukhetha. uvavanyo lwe-enzyme kunye novavanyo lophando.
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I-Dipso sodium CAS: 102783-62-0 Ixabiso lomvelisi
I-3--[N, N-Bis (i-hydroxyethyl) i-amino] -2-hydroxypropanesulphonic acid ityuwa yesodium, eyaziwa ngokuba yi-BES ityuwa ye-sodium, i-chemical compound esetyenziswa ngokuqhelekileyo kuphando lwe-biochemical kunye nezicelo zamachiza.I-sulfonic acid derivative kunye nefom yetyuwa ye-sodium, eyenza i-soluble yamanzi kwaye izinzile kwizisombululo ezinamanzi.
I-BES ityuwa ye-sodium ine-molecular formula ye-C10H22NNaO6S kunye nobunzima be-molecular malunga ne-323.34 g / mol.Ihlala isetyenziswa njenge-agent ephazamisayo ngenxa yokukwazi ukugcina uluhlu lwe-pH oluzinzileyo kwizisombululo.
Le khompawundi yaziwa ngokukwazi kwayo ukumelana notshintsho lwe-pH olubangelwa kukuhlanjululwa okanye ukongezwa kweeasidi kunye neziseko.Iqhele ukusetyenziswa kwiziphumo zebhayoloji kunye ne-enzymatic, imidiya yenkcubeko yeeseli, ukucocwa kweeprotheyini, kunye nezinye iinkqubo apho ulawulo oluchanekileyo lwe-pH lubalulekile.
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I-Bis-tris hydrochloride CAS: 124763-51-5
I-Bis-tris hydrochloride yikhompawundi eneempawu zokuthintela ezidla ngokusetyenziswa kwimifuniselo yebhayoloji neyebhayoloji.Inceda ukugcina i-pH ezinzileyo kwaye isetyenziswe kwiprotheyini ye-electrophoresis, i-enzyme assays, inkcubeko yeseli, kunye nokuqulunqwa kwamayeza.Umsebenzi wayo oyintloko kukuxhathisa utshintsho kwi-pH xa i-acids okanye iziseko zongezwa kwisisombululo, okwenza kube sisixhobo esibalulekileyo kwizicelo ezahlukeneyo zesayensi kunye nezoshishino.
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I-4-Nitrophenyl-beta-D-glucopyranoside CAS: 2492-87-7
I-4-Nitrophenyl-beta-D-glucopyranoside i-substrate esetyenziswa ngokuqhelekileyo kwiimvavanyo ze-biochemical ukuhlola umsebenzi wee-enzymes ezifana ne-β-glucuronidase.Le ngqungquthela i-hydrolyzed yi-enzyme, ebangela ukukhululwa kwe-4-nitrophenol, enokulinganiswa ngokusebenzisa i-spectrophotometry.Ukusetyenziswa kwayo kwenza abaphandi bafunde iinkalo ezahlukeneyo zemetabolism yeziyobisi, i-toxicology, kunye noxilongo lweklinikhi olunxulumene nokuphendula kwe-glucuronidation.
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Tris Base CAS: 77-86-1 Ixabiso lomvelisi
I-Tris Base, eyaziwa ngokuba yi-Tromethamine okanye i-THAM, yikhompawundi ye-organic edla ngokusetyenziswa kwinkalo ye-biochemistry kunye ne-molecular biology.Ngumgubo omhlophe, oyikristale onyibilika kakhulu emanzini kwaye unevumba le-amine.I-Tris Base isoloko isetyenziswa njenge-agent ephazamisayo ukugcina i-pH ezinzileyo kwiimvavanyo ezahlukeneyo zebhayoloji kunye neenkqubo, ezifana ne-DNA kunye nezifundo zeprotheni.Inokusetyenziswa kwakhona ekuqulunqweni kwamachiza kunye nokuveliswa kwee-agent ezisebenza phezulu.Lilonke, i-Tris Base licandelo elibalulekileyo kwizicelo ezininzi zaselabhoratri apho ukugcinwa kwe-pH echanekileyo kubalulekile.
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I-Heppso sodium CAS: 89648-37-3 Ixabiso lomvelisi
N- [2-Hydroxyethyl]piperazine-N'-[2-hydroxypropanesulfonic acid] ityuwa yesodium yi-chemical compound kunye ne-formula C8H19N2NaO4S.Ityuwa yesodium ephuma kwi-piperazine, equkethe i-hydroxyethyl kunye ne-hydroxypropanesulfonic acid functional groups.Isetyenziswa ngokuqhelekileyo kwishishini loxubo-mayeza njenge-agent ekhuselayo kunye nezinzisi kuqulunqo lwamachiza.Le khompawundi inceda ukugcina i-pH kunye nokuzinza kwamayeza.
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1,2,3,4,6-penta-O-acetyl-alpha-D-galactopyranose CAS:4163-59-1
I-1,2,3,4,6-penta-O-acetyl-alpha-D-galactopyranose yikhemikhali yekhemikhali yentsapho ye-carbohydrates.Iphuma kwi-alpha-D-galactopyranose, iswekile yendalo.Le khompawundi ethile inamaqela amahlanu e-acetyl eqhotyoshelwe kumaqela athile e-hydroxyl kwi-molecule yeswekile.Isetyenziswa ngokuqhelekileyo kwizicelo ezahlukeneyo zeekhemikhali kunye namayeza, kubandakanywa njengesixhobo sokuqala sokuhlanganiswa kwezinye iikhompawundi.Ifom yayo ye-acetylated iphucula ukuzinza kwayo kunye nokusebenza kwakhona, okwenza kube yinto ebalulekileyo yokwakha kwi-organic chemistry.
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popso sesquisodium CAS:108321-08-0
I-Piperazine-N,N'-bis(2-hydroxypropanesulfonic acid) ityuwa ye-sesquisodium, ekwaziwa ngokuba yi-PIPES sesquisodium ityuwa, yikhompawundi yemichiza esetyenziswa njenge-agent ekhuselayo kwiinkqubo ezahlukeneyo zenzululwazi kunye nezoshishino.Inceda ukugcina izinga le-pH elizinzileyo kwizisombululo, ngakumbi kuluhlu lwe-physiological pH.I-PIPES sesquisodium ityuwa iqhele ukusetyenziswa kumajelo eendaba enkcubeko yeeseli, iprotein biochemistry, i-electrophoresis, ubuchule bebhayoloji yemolekyuli, kunye neenkqubo zokuhanjiswa kweziyobisi.Isebenza njengomlawuli we-pH kunye nesixhasi somsebenzi we-enzyme kunye nokuzinza, okwenza ukuba ixabiseke kuluhlu olubanzi lweenkqubo zophando kunye nezoshishino.
