I-P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

I-P-Nitrophenyl beta-D-lactopyranoside, ekwaziwa ngokuba yi-PNPG, yikhompawundi esetyenziswa rhoqo kwiimvavanyo ze-enzyme ukulinganisa umsebenzi we-beta-galactosidase, i-enzyme ebandakanyeka kwi-carbohydrate metabolism. I-PNPG yi-substrate yokwenziwa enokuqhekeka yi-beta-galactosidase, nto leyo ekhokelela ekukhutshweni kwemveliso enombala otyheli. Ubungakanani be-substrate hydrolysis bunokulinganiswa nge-spectrophotometric ngokulinganisa ukufunxwa kwemveliso kubude obuthile. Oku kuvumela abaphandi ukuba bavavanye umsebenzi kunye ne-kinetics ye-beta-galactosidase kwiimeko ezahlukeneyo, ezinje ngokufunda umsebenzi we-enzyme, ukuhlola ii-enzyme inhibitors okanye ii-activators, okanye ukuvavanya imiphumo ye-mutations kumsebenzi we-enzyme.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukuchonga umsebenzi we-beta-galactosidase: I-PNPG isetyenziswa rhoqo kwiimvavanyo ukulinganisa umsebenzi we-beta-galactosidase, i-enzyme ekhuthaza i-hydrolysis ye-lactose ibe yi-glucose kunye ne-galactose. I-hydrolysis ye-PNPG yi-beta-galactosidase ikhupha imolekyuli ye-p-nitrophenol (pNP), enokufunyanwa nge-spectrophotometric ngenxa yombala wayo otyheli.

Ukuhlolwa kwee-enzyme inhibitors kunye nee-activators: I-PNPG ingasetyenziswa ekuhlolweni okuphezulu ukuze kuchongwe iikhompawundi ezilawula umsebenzi we-beta-galactosidase. Ngokulinganisa izinga le-PNPG hydrolysis phambi kweekhompawundi ezahlukeneyo zovavanyo, abaphandi banokuchonga ii-inhibitors ezinciphisa umsebenzi we-enzyme okanye ii-activators eziphucula umsebenzi we-enzyme.

Uphononongo lwe-enzyme kinetics: I-hydrolysis ye-PNPG yi-beta-galactosidase ilandela i-Michaelis-Menten kinetics, evumela abaphandi ukuba bamisele iiparameter ezibalulekileyo ze-enzyme ezifana ne-maximum reaction velocity (Vmax) kunye ne-Michaelis constant (Km). Olu lwazi lunceda ekuqondeni i-substrate affinity ye-enzyme kunye nokusebenza kakuhle kwe-catalytic.

Ukusetyenziswa kwebhayoloji yemolekyuli: I-Beta-galactosidase, eqhekeza i-PNPG, isetyenziswa kakhulu njengejini yentatheli kwibhayoloji yemolekyuli. I-substrate ye-PNPG idla ngokusetyenziselwa ukubona nokubona ukubonakaliswa kwejini yentatheli, inika indlela elula neyobuchule yokuvavanya ukubonakaliswa kwejini kwiinkqubo ezahlukeneyo zovavanyo.

Isampuli yeMveliso

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C18H25NO13
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	4419-94-7
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.