I-Phenylgalactoside, ekwabizwa ngokuba yi-p-nitrophenyl β-D-galactopyranoside (pNPG), yinxalenye yokwenziwa esetyenziswa rhoqo kuvavanyo lwebhayoloji yebhayoloji kunye nemolekyuli.Ngokuqhelekileyo isetyenziselwa ukufumanisa nokulinganisa umsebenzi we-enzyme β-galactosidase.
Xa i-phenylgalactoside i-hydrolyzed yi-β-galactosidase, ikhupha i-p-nitrophenol, i-compound enemibala ephuzi.Ukukhululwa kwe-p-nitrophenol kunokulinganiswa ngokobungakanani kusetyenziswa i-spectrophotometer, njengoko i-absorbection ye-p-nitrophenol inokubonwa kwi-wavelength ye-405 nm.