I-Tris maleate CAS:72200-76-1

Umthamo wokufaka i-buffering: I-Tris (i-maleate) yi-pH buffer esebenzayo, oko kuthetha ukuba inokumelana notshintsho kwi-pH ngokufunxa okanye ukukhulula iiproton. Isetyenziswa kakhulu ukugcina uluhlu oluthile lwe-pH, oluqhele ukuba phakathi kwe-pH 6 kunye ne-8, kwiinkqubo ezahlukeneyo zebhayoloji kunye neekhemikhali.

Uphando lweeproteni kunye neeenzyme: I-Tris (i-maleate) isetyenziswa rhoqo kwizifundo zeeproteni kunye neeenzyme, apho ukugcina i-pH ezinzileyo kubalulekile ekugcineni uzinzo kunye nomsebenzi wazo. Inokunceda ukugcina ukwakheka kunye nomsebenzi weeproteni ngokuthintela ukuguquka kwe-pH okubangelwa yi-pH.

Ukusetyenziswa kwebhayoloji yemolekyuli: I-Tris (i-maleate) ikwasetyenziswa kakhulu kwiindlela zebhayoloji yemolekyuli ezifana ne-DNA kunye ne-RNA isolation, i-polymerase chain reaction (PCR), kunye ne-gel electrophoresis. Inceda ukugcina iimeko ze-pH ezifanelekileyo ezifunekayo kwezi nkqubo kwaye iqinisekisa ukuchaneka kwazo kunye nokuphindaphinda kwazo.

Ukusetyenziswa kwemizi-mveliso: I-Tris (i-maleate) isetyenziswa kwiinkqubo ezahlukeneyo zoshishino ezifana nokuveliswa kwamayeza, ukubiliswa, kunye ne-biotechnology. Isetyenziselwa ukulawula i-pH kwimveliso enkulu, ukuqinisekisa iimeko ezifanelekileyo zokukhula kunye nomsebenzi weentsholongwane okanye ukwenziwa kweemveliso ezifunwayo.

Ikhemistri yohlalutyo: I-Tris (i-maleate) isetyenziswa kwikhemistri yohlalutyo lokulinganisa kunye nokulinganisa i-pH metres, kunye nokulungiselela ii-calibration buffers zokulinganisa i-pH. Inika ixabiso le-pH elaziwayo lokulinganisa ngokuchanekileyo nangokuthembekileyo.